Anaphylactic and non-anaphylactic murine IgG1 differ in their ability to bind to mast cells: relevance of proper glycosylation of the molecule

Immunobiology
Eliana L Faquim-MauroMahasti S Macedo

Abstract

We have previously shown that murine IgG1 antibodies comprise two functionally distinct types regarding their ability to induce mast cell degranulation. In this work, we identified two IgG1-producing hybridomas, both with the same antigenic specificity (anti-DNP), but different in vivo anaphylactic activities. Whereas one of them secretes the anaphylactic IgG1 antibody, as assessed by passive cutaneous anaphylaxis, the other produces the non-anaphylactic IgG1 molecule. The evaluation of the ability of both types of IgG1 to bind to and activate a mouse mast cell line revealed that the anaphylactic IgG1 has a higher binding capacity and releases more beta-hexosaminidase from mast cells than the non-anaphylactic IgG1. Aglycosylated IgG1 obtained by treatment of the anaphylactic IgG1-producing hybridoma line with an inhibitor of N-glycosylation failed to elicit anaphylaxis. In addition, a goat anti-mouse IgG1 antibody reacted less with this aglycosylated IgG1 than with the glycosylated form. These results suggest that the anaphylactic activity of IgG1 antibodies is closely related to their structural conformation and the proper N-glycosylation of these molecules. Finally, the difference in the anaphylactic property between the two ...Continue Reading

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Citations

Jun 29, 2006·International Archives of Allergy and Immunology·Aldacilene S SilvaMahasti S Macedo
May 15, 2007·Immunological Reviews·Odile Malbec, Marc Daëron
Jul 9, 2004·The Journal of Allergy and Clinical Immunology·Maria Fernanda Macedo-SoaresMahasti S Macedo
Jun 16, 2005·International Archives of Allergy and Immunology·Claudia Milena Trujillo-VargasKlaus Joseph Erb
Dec 19, 2003·Current Allergy and Asthma Reports·Ali A Siddiqui, Philip B Miner
Dec 11, 2008·Clinical and Experimental Medicine·Hai-Ling QiaoXin Tian
May 17, 2007·Current Allergy and Asthma Reports·M Luisa Baeza, José Manuel Zubeldia
Sep 2, 2005·The Korean Journal of Internal Medicine·Yoon-Seok ChangYou-Young Kim
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandriana R SilvaEliana L Faquim-Mauro
Nov 26, 2010·The Journal of Allergy and Clinical Immunology·Mike KulisWesley Burks

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