Anaphylatoxin-like molecules generated during complement activation induce a dramatic enhancement of particle uptake in rainbow trout phagocytes

Developmental and Comparative Immunology
J LiJ O Sunyer

Abstract

Here we have identified a serum fraction containing approximately 8-kDa molecules with an unexpected capacity to greatly enhance particle uptake in trout head kidney leukocytes (HKLs). This approximately 8-kDa particle-uptake enhancing fraction (PUEF-8) was purified from complement-activated serum by gel filtration chromatography. Mass spectrometric analysis and reactivity of anti-trout C3-1 and C4 antibodies, indicated the presence of C3a, C4a and C5a molecules in PUEF-8. Using a newly developed flow cytometric assay that measures the capacity of cells to ingest fluorescent beads, we showed that PUEF-8 induced a striking enhancement (344+/-50% higher than the PBS control value) in the number of HKLs ingesting three or more beads. In contrast, the effect of PUEF-8 on peripheral blood leukocytes (PBLs) was almost negligible. Interestingly, PUEF-8 acted as a strong chemoattractant for both HKLs and PBLs. These findings suggest a novel role for the anaphylatoxins generated during complement activation in teleost fish.

References

Jan 1, 1991·Pharmaceutical Research·J F ScieszkaM J Cho
Jan 1, 1984·Springer Seminars in Immunopathology·T E Hugli
Jan 1, 1980·International Archives of Allergy and Applied Immunology·B DamerauW Vogt
Jul 1, 1994·European Journal of Immunology·B BürgiC A Dahinden
Jul 1, 1994·Developmental and Comparative Immunology·R A NewtonC L Geczy
Jul 1, 1996·Scandinavian Journal of Immunology·T WerfelJ Zwirner
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J O SunyerJ D Lambris
Jan 1, 1997·Seminars in Liver Disease·K Whaley, W Schwaeble
Feb 26, 1998·Immunopharmacology·J A Ember, T E Hugli
Nov 4, 1998·Seminars in Immunology·D T Fearon
Nov 18, 1998·Immunology Today·J O SunyerJ D Lambris
Jan 23, 1999·Immunological Reviews·J O Sunyer, J D Lambris
Jan 23, 1999·Immunological Reviews·S L Smith
Aug 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·S M VogenS A Sherman
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J T UlrichS D Sanderson
Sep 12, 2000·Developmental and Comparative Immunology·I K ZarkadisJ D Lambris
Sep 15, 2000·Journal of Immunological Methods·A K LehmannA Halstensen
Jan 13, 2001·Current Opinion in Immunology·M Nonaka
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L KacaniM P Dierich
May 18, 2001·Developmental and Comparative Immunology·S FranchiniJ D Lambris
May 18, 2001·Developmental and Comparative Immunology·J L StaffordM Belosevic
Jun 21, 2001·Immunological Reviews·L C SmithD P Terwilliger
Aug 11, 2001·Veterinary Immunology and Immunopathology·S HongC J Secombes
Jun 24, 2003·Developmental and Comparative Immunology·J ZouC J Secombes
Sep 19, 2007·Current Opinion in Immunology·Chris T AmemiyaAgustin Zapata

❮ Previous
Next ❯

Citations

Nov 8, 2016·Fish & Shellfish Immunology·Francesca D'EsteMarco Scocchi
Nov 9, 2007·Developmental and Comparative Immunology·Leon GrayferMiodrag Belosevic
Jun 13, 2018·Frontiers in Immunology·Leon GrayferMiodrag Belosevic
Jun 30, 2019·Developmental and Comparative Immunology·Wenche JohansenKnut Olav Strætkvern
Nov 7, 2019·Fish & Shellfish Immunology·Qingyun YangHongyan Li
Nov 6, 2007·Fish & Shellfish Immunology·Shona K Whyte

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.