Jun 10, 2000

Anaphylaxis to chlorhexidine. Case report. Implication of immunoglobulin E antibodies and identification of an allergenic determinant

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
N H PhamB A Baldo


There are many reports of allergic reactions, including anaphylaxis, following exposure to chlorhexidine. Reactions may occur via contact with the skin and mucous membranes or from catheters treated with the antibacterial agent. Apart from implicating chlorguanide in immunoglobulin (Ig) E antibody-binding studies on serum from an anaphylactic patient, little work has been done on the molecular basis of recognition of the agent in sensitive subjects. The molecular basis of IgE-binding to chlorhexidine was closely examined with the view of defining its fine structural recognition features by antibodies from a subject who experienced anaphylaxis following contact with the antiseptic. Tryptase determinations, different drug-solid phases, immunoassays and quantitative hapten inhibition studies with chlorhexidine and selected structural analogues were employed together with serum from the anaphylactic patient. Results were analysed to define the complete drug allergenic determinant and to identify the important structural features complementary to the IgE antibody combining sites. The subject's serum tryptase levels sampled after the reaction were elevated and employment of a chlorhexidine-EA Sepharose solid phase showed the presence...Continue Reading

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Mentioned in this Paper

Immunoglobulin E Measurement
Anaphylaxis (Non Medication)
Immunoglobulin Activity
Tryptase Measurement
Binding Sites, Antibody
Antigenic Specificity
Cessation of Life

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Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.