AnaSP: a software suite for automatic image analysis of multicellular spheroids

Computer Methods and Programs in Biomedicine
Filippo Piccinini

Abstract

Today, more and more biological laboratories use 3D cell cultures and tissues grown in vitro as a 3D model of in vivo tumours and metastases. In the last decades, it has been extensively established that multicellular spheroids represent an efficient model to validate effects of drugs and treatments for human care applications. However, a lack of methods for quantitative analysis limits the usage of spheroids as models for routine experiments. Several methods have been proposed in literature to perform high throughput experiments employing spheroids by automatically computing different morphological parameters, such as diameter, volume and sphericity. Nevertheless, these systems are typically grounded on expensive automated technologies, that make the suggested solutions affordable only for a limited subset of laboratories, frequently performing high content screening analysis. In this work we propose AnaSP, an open source software suitable for automatically estimating several morphological parameters of spheroids, by simply analyzing brightfield images acquired with a standard widefield microscope, also not endowed with a motorized stage. The experiments performed proved sensitivity and precision of the segmentation method pro...Continue Reading

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Citations

Oct 13, 2016·Scientific Reports·Liliana R Cisneros CastilloAnne Régnier-Vigouroux
Sep 7, 2017·Anais Da Academia Brasileira De Ciências·Juliana O DE CarvalhoAndré L Tambara
Feb 23, 2020·Anti-cancer Drugs·Laurita BoffCláudia Maria Oliveira Simões
Oct 28, 2019·Scientific Reports·Maria HarmatiKrisztina Buzas
Aug 18, 2017·Biological Procedures Online·Filippo PiccininiAlessandro Bevilacqua
Jan 19, 2021·Biotechnology Progress·Gatika AgrawalMaddaly Ravi
Nov 23, 2020·Computer Methods and Programs in Biomedicine·David LacalleIgnacio Ochoa
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fernanda MalhãoAlice Abreu Ramos
Jul 22, 2021·Scientific Reports·Istvan GrexaPeter Horvath
Dec 7, 2021·Frontiers in Molecular Biosciences·Jose R Aguilar CosmeFrederik Claeyssens

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