Anatomical Evidence for a Direct Projection from Purkinje Cells in the Mouse Cerebellar Vermis to Medial Parabrachial Nucleus

Frontiers in Neural Circuits
Mitsuhiro HashimotoHiroyuki Yaginuma

Abstract

Cerebellar malformations cause changes to the sleep-wake cycle, resulting in sleep disturbance. However, it is unclear how the cerebellum contributes to the sleep-wake cycle. To examine the neural connections between the cerebellum and the nuclei involved in the sleep-wake cycle, we investigated the axonal projections of Purkinje cells in the mouse posterior vermis by using an adeno-associated virus (AAV) vector (serotype rh10) as an anterograde tracer. When an AAV vector expressing humanized renilla green fluorescent protein was injected into the cerebellar lobule IX, hrGFP and synaptophysin double-positive axonal terminals were observed in the region of medial parabrachial nucleus (MPB). The MPB is involved in the phase transition from rapid eye movement (REM) sleep to Non-REM sleep and vice versa, and the cardiovascular and respiratory responses. The hrGFP-positive axons from lobule IX went through the ventral spinocerebellar tract and finally reached the MPB. By contrast, when the AAV vector was injected into cerebellar lobule VI, no hrGFP-positive axons were observed in the MPB. To examine neurons projecting to the MPB, we unilaterally injected Fast Blue and AAV vector (retrograde serotype, rAAV2-retro) as retrograde trace...Continue Reading

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Citations

Nov 5, 2019·The Journal of Physiology·Martha L Streng, Esther Krook-Magnuson
Jul 16, 2020·Journal of Neuroscience Methods·Adriana K CushnieSarah R Heilbronner
May 16, 2021·Sleep & Breathing = Schlaf & Atmung·Bijia Song, Junchao Zhu
Jul 13, 2021·Frontiers in Neuroscience·Bijia Song, Jun-Chao Zhu
Feb 10, 2020·Epilepsy & Behavior : E&B·Martha L Streng, Esther Krook-Magnuson

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Methods Mentioned

BETA
PCR
confocal microscopy
genetic modification

Software Mentioned

FLUOVIEW
Photoshop
ImageJ

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