PMID: 3747591Jul 1, 1986Paper

Anatomically selective peripheral nerve ablation using intraneural ricin injection

Journal of Neuroscience Methods
R G Wiley, T N Oeltmann

Abstract

Anatomically selective destruction of sensory and motor neurons based upon which nerve contains the corresponding axons can be accomplished by intraneural pressure microinjection of the toxic lectin, ricin. Ricin is taken up by axons at the injection site and axonally transported to perikarya resulting in destruction of the neurons. In the present report, we describe a reliable procedure for making such lesions using pressure microinjection of ricin into nerve trunks. Consistent, complete lesions restricted to the appropriate sensory and motor neurons are documented after injection of the vagus, hypoglossal, phrenic and sciatic nerves and the superior cervical ganglion. Complete vagal ablations could be achieved with 100 ng or less of ricin; whereas, 1-3 micrograms was required to obtain similar results with hypoglossal and sciatic nerves. Although most neurons are dead within 24 h after the injection, survival times of 10-14 days may be necessary for complete disappearance of poisoned neurons. This technique can be valuable in making highly selective lesions for anatomical, neurochemical and neurophysiological experiments.

References

Nov 1, 1979·Journal of Toxicology and Environmental Health·O FodstadA Pihl
Sep 1, 1985·Journal of Neuropathology and Experimental Neurology·R G WileyG T Keusch
Oct 1, 1985·Journal of the Neurological Sciences·T YamamotoH Kudo
Oct 21, 1985·Brain Research·L R JohnsonR C Canfield
Jan 1, 1971·International Review of Neurobiology·A R Lieberman
Jun 20, 1983·Brain Research·R G WileyD J Reis
Jan 1, 1984·Journal of Neurosurgery·T YamamotoH Konno
Sep 30, 1982·Biochemical and Biophysical Research Communications·W P MacConnellN O Kaplan

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Citations

Jun 1, 1987·Journal of Neurocytology·E A Ling, S K Leong
Apr 1, 1989·Journal of Neuroscience Methods·R G Wiley, T N Oeltmann
Nov 1, 1994·Journal of Neuroscience Methods·H Z TangR Hammerschlag
Aug 22, 2008·Injury·Andreas F MavrogenisPanayotis N Soucacos
Dec 24, 1990·Brain Research·C G CusickR G Wiley
Oct 27, 2009·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Wei-Zai ShenWing-Hang Kwong
Feb 8, 1988·The Journal of Comparative Neurology·W J Streit, G W Kreutzberg
Dec 16, 2020·Neural Regeneration Research·Allison S LiangRandall D McKinnon
Jul 30, 2018·Journal of Neuroscience Methods·Yajie LiangJeff W M Bulte
Dec 21, 1993·Biochimica Et Biophysica Acta·L BarbieriF Stirpe
Sep 8, 1988·The Journal of Comparative Neurology·L M Pubols, M E Foglesong

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