PMID: 8606800Nov 1, 1995Paper

Anatoxin-a-evoked [3H]dopamine release from rat striatal synaptosomes

Neuropharmacology
L SoliakovS Wonnacott

Abstract

Presynaptic nicotinic acetylcholine receptors on striatal nerve terminals modulate the release of dopamine. Using rat striatal synaptosomes loaded with [3H]dopamine, we have characterized the action of the selective nicotinic agonist, (+/-)anatoxin-a, with respect to [3H]dopamine release, in order to explore the mechanisms coupling nicotinic receptor activation to exocytosis. Anatoxin-a evoked [3H]dopamine release in a concentration-dependent and mecamylamine-sensitive manner, EC50 = 0.11 microM. The maximum [3H]dopamine release elicited by anatoxin-a was only 20% of the maximum elicited by KCl depolarization; there was no additivity between anatoxin-a and sub-maximal concentrations of KCl. Both agents stimulated Ca(2+)-dependent release that was equally sensitive to inhibition by 200 microM Cd2+. This result suggests that anatoxin-a-stimulated exocytosis is mediated by Ca2+ influx via voltage-sensitive Ca2+ channels, with little contribution from Ca2+ entering directly through the nicotinic receptor channel. This view is supported by the abolition of anatoxin-a-evoked [3H]dopamine release in Na(+)-depleted medium. A partial (40%) inhibition by tetrodotoxin was observed. These data suggest that activation of presynaptic nicotin...Continue Reading

References

Jan 1, 1978·Biochemical Pharmacology·L ArquerosE Zunino
Dec 1, 1984·Journal of Neurochemistry·P P Rowell, D L Winkler
Dec 1, 1993·Pflügers Archiv : European journal of physiology·Z Zhou, E Neher
May 1, 1993·Biochemical Society Transactions·G I WilkieS Wonnacott
Jun 1, 1993·Journal of Neurochemistry·P ThomasE X Albuquerque
Jan 1, 1985·Neurochemistry International·C RapierS Wonnacott

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Citations

Aug 10, 2006·Journal of Neural Transmission·F CamposM Alfonso
Apr 20, 2000·European Journal of Pharmacology·S WonnacottI W Jones
Jan 7, 1999·European Journal of Pharmacology·P T Toth, E S Vizi
Nov 14, 2002·Neurotoxicology·Maryka Quik, Jennifer M Kulak
Feb 1, 1997·Trends in Neurosciences·S Wonnacott
Jul 6, 2000·Archives of Medical Research·S Mihailescu, R Drucker-Colín
Nov 19, 1997·Progress in Neurobiology·C GottiF Clementi
Jun 1, 1996·Current Opinion in Neurobiology·D S McGehee, L W Role
Oct 1, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Carrie K JonesMichael Bubser
Jan 8, 2009·Physiological Reviews·Edson X AlbuquerqueScott W Rogers
Feb 18, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Subham BanerjeeVijay Veer
Jan 1, 1996·Neuropharmacology·D MarshallS Wonnacott
Aug 4, 2007·Environment International·Joana OsswaldVitor Vasconcelos
Apr 5, 2008·Neurotoxicology and Teratology·K A JaremaR C MacPhail
Dec 31, 2014·European Journal of Drug Metabolism and Pharmacokinetics·Subham BanerjeeVijay Veer
Apr 1, 2003·Environmental Toxicology and Pharmacology·András PappIllés Dési
Apr 7, 2007·Neuropharmacology·Anne-Sophie VillégierFrances M Leslie
Jul 6, 2017·The European Journal of Neuroscience·Asami TanimuraDalton James Surmeier
Jul 3, 1999·Physiological Reviews·A MeirR Rahamimoff
Jun 8, 2006·Neurochemical Research·F CamposM Alfonso
Mar 7, 2020·Acta Pharmacologica Sinica·Changliang Liu
Jan 22, 1998·Journal of Medicinal Chemistry·M W HolladayJ K Lynch

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