PMID: 6991508Feb 1, 1980Paper

Anchorage independent growth and plasminogen activator production by bovine endothelial cells

The Journal of Cell Biology
W E LaugN Sorgente

Abstract

Endothelial cells obtained from the aortae of 1- to 2-d-old calves were cloned at high efficiency using fibrin-coated dishes. Primary cultures as well as clones derived from them produced high fibrinolytic activity when grown on 125I-fibrin-coated dishes which was 90% dependent upon the presence of plasminogen. High plasminogen-dependent proteolytic activity was also demonstrated in endothelial cell lysates and in the culture medium of the cells. The production and secretion of the plasminogen activator(s) were found to increase during the log phase of cell growth and to reach a maximum level at confluence. These endothelial cells exhibited morphological phenotypes comparable to those of transformed cells when grown in the presence of acid-treated fetal calf, dog, or human serum. Furthermore, they demonstrated anchorage independent growth, and large colonies were formed in semisolid media. Spontaneous neoplastic transformation of these cells was excluded by karyotypic analysis, lack of tumorigenicity in athymic nude mice, and limited lifespan in culture. Cell clones isolated from colonies grown in agarose demonstrated the same growth characteristics and proteolytic activity as before plating in agarose. High fibrinolytic activi...Continue Reading

References

May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·V Buonassisi, J C Venter
Oct 30, 1971·Lancet·M Seabright
Jun 1, 1966·The Australian Journal of Experimental Biology and Medical Science·T R Bradley, D Metcalf
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R PollackD Rifkin
Apr 16, 1973·Humangenetik·B Paul, I H Porter
Apr 1, 1974·The Journal of Experimental Medicine·J C UnkelessE Reich
May 1, 1970·The Journal of Cell Biology·A L Horwitz, A Dorfman
Jul 1, 1959·The Journal of Pathology and Bacteriology·A S TODD
Feb 26, 1962·Biochimica Et Biophysica Acta·M W MOSESSON
May 17, 1947·Nature·T ASTRUP, P M PERMIN

❮ Previous
Next ❯

Citations

May 1, 1983·In Vitro·C M Gajdusek, S M Schwartz
Jan 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·P J La Rocca, J G Rheinwald
Nov 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J C Bowersox, N Sorgente
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D M Peehl, E J Stanbridge
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J LoskutoffD Lawrence
Dec 1, 1982·The Journal of Cell Biology·J L GrossD B Rifkin
Dec 4, 1992·Annals of the New York Academy of Sciences·C L Jackson, M A Reidy
Mar 1, 1989·British Journal of Industrial Medicine·S T Ho, H S Yu
Jan 1, 1984·Experimental Lung Research·M Schuyler, W Forman
Jan 1, 1991·Acta Oncologica·C MothersillT Hennessy
Mar 1, 1983·Journal of Cellular Physiology·J B GrinspanE M Levine
Dec 1, 1985·Journal of Cellular Physiology·S RogneE O Pettersen
Nov 1, 1983·The Journal of Pathology·A M Schor, S L Schor
Jan 27, 1983·Biochemical and Biophysical Research Communications·J J EmeisG Wijngaards
Mar 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S M SchwartzS C Selden
Jan 1, 1991·Molecular Carcinogenesis·F BasoloA J Klein-Szanto
Jan 1, 1985·Biochimica Et Biophysica Acta·J M Vasiliev

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.