Anchoring mechanisms of membrane-associated M13 major coat protein

Chemistry and Physics of Lipids
David StoparMarcus A Hemminga

Abstract

Bacteriophage M13 major coat protein is extensively used as a biophysical, biochemical, and molecular biology reference system for studying membrane proteins. The protein has several elements that control its position and orientation in a lipid bilayer. The N-terminus is dominated by the presence of negatively charged amino acid residues (Glu2, Asp4, and Asp5), which will always try to extend into the aqueous phase and therefore act as a hydrophilic anchor. The amphipathic and the hydrophobic transmembrane part contain the most important hydrophobic anchoring elements. In addition there are specific aromatic and charged amino acid residues in these domains (Phe 11, Tyr21, Tyr24, Trp26, Phe42, Phe45, Lys40, Lys43, and Lys44) that fine-tune the association of the protein to the lipid bilayer. The interfacial Tyr residues are important recognition elements for precise protein positioning, a function that cannot be performed optimally by residues with an aliphatic character. The Trp26 anchor is not very strong: depending on the context, the tryptophan residue may move in or out of the membrane. On the other hand, Lys residues and Phe residues at the C-terminus of the protein act in a unique concerted action to strongly anchor the p...Continue Reading

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Citations

Aug 15, 2009·European Biophysics Journal : EBJ·Marcus A HemmingaDavid Stopar
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Justin L LorieauAd Bax
Jul 30, 2010·The Journal of Biological Chemistry·Vitaly V VostrikovRoger E Koeppe
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Apr 3, 2010·Chemistry and Physics of Lipids·Ivana KomljenovićEdward Sternin
Apr 14, 2009·Trends in Biochemical Sciences·Werner L VosMarcus A Hemminga
Jan 14, 2009·Journal of Magnetic Resonance·Janez StrancarMarcus A Hemminga
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Jan 21, 2014·Biochimica Et Biophysica Acta·Neil R HariaChristian D Lorenz
Aug 15, 2006·Biophysical Journal·David StoparMarcus A Hemminga
Aug 21, 2007·Biophysical Journal·Werner L VosMarcus A Hemminga
Sep 1, 2009·Biochimica Et Biophysica Acta·David StoparMarcus A Hemminga
Aug 9, 2018·Journal of Lipid Research·Diego E SastreSilvia G Altabe

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