Anchoring of histidine-tagged proteins to molecular printboards: self-assembly, thermodynamic modeling, and patterning

Chemistry : a European Journal
Manon J W LuddenJurriaan Huskens

Abstract

In this paper the multivalent binding of hexahistidine (His6)-tagged proteins to beta-cyclodextrin (beta-CD) self-assembled monolayers (SAMs) by using the nickel(II) complex of a hetero-divalent orthogonal adamantyl nitrilotriacetate linker (4) is described. Nonspecific interactions were suppressed by using monovalent adamantyl-hexa(ethylene glycol) derivative 3. With the mono-His6-tagged maltose binding protein (His6-MBP), thermodynamic modeling based on surface plasmon resonance (SPR) titration data showed that the MBP molecules in solution were linked, on average, to Ni.4 in 1:1 stoichiometry. On the surface, however, the majority of His(6)-MBP was complexed to surface-immobilized beta-CDs through three Ni.4 complexes. This difference is explained by the high effective beta-CD concentration at the surface and is a new example of supramolecular interfacial expression. In a similar adsorption scheme, SPR proved that the alpha-proteasome could be attached to beta-CD SAMs in a specific manner. Patterning through microcontact printing of (His6)4-DsRed-fluorescent timer (DsRed-FT), which is a tetrameric, visible autofluorescent protein, was carried out in the presence of Ni.4 Fluorescence measurements showed that the (His6)4-DsRed...Continue Reading

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·C DietrichR Tampé
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·W Boos, H Shuman
Apr 2, 1999·Biosensors & Bioelectronics·D KrögerH Vogel
May 18, 1999·Journal of Molecular Biology·I T DornR Tampé
Apr 29, 2000·Chemistry : a European Journal·M W BeulenD N Reinhoudt
Feb 19, 2002·Chemical Reviews·Mikhail V. Rekharsky, Yoshihisa Inoue
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gang-Yu Liu, Nabil A Amro
Jul 13, 2002·The Journal of Biological Chemistry·Andreas ThessReinhard Guckenberger
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jörg WiedenmannG Ulrich Nienhaus
Sep 27, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mikhail V MatzSergey A Lukyanov
Nov 30, 2002·Angewandte Chemie·Jurriaan HuskensDavid N Reinhoudt
Jan 6, 2004·Angewandte Chemie·Tommaso AulettaDavid N Reinhoudt
Mar 3, 2004·Nature Biotechnology·Vladislav V Verkhusha, Konstantin A Lukyanov
May 27, 2004·Journal of the American Chemical Society·Jurriaan HuskensDavid N Reinhoudt
Jun 4, 2004·The EMBO Journal·Silke HutschenreiterRobert Tampé
Jul 16, 2004·Analytical Chemistry·Electra Gizeli, Johanna Glad
Apr 21, 2005·Journal of the American Chemical Society·Naoufel HaddourChantal Gondran
Apr 22, 2005·Chemistry : a European Journal·Christiaan M BruininkDavid N Reinhoudt
May 12, 2005·Chemical Communications : Chem Comm·Harald Hofmeier, Ulrich S Schubert
Jul 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Steffen OnclinDavid N Reinhoudt
Jul 21, 2005·Journal of the American Chemical Society·Suman LataJacob Piehler
Jul 27, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Koichi KatoHiroo Iwata
Feb 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Roland GamsjaegerChristoph Romanin
Feb 16, 2006·Journal of the American Chemical Society·Suman LataJacob Piehler
Aug 5, 2006·Chembiochem : a European Journal of Chemical Biology·Ramūnas ValiokasJacob Piehler
Dec 21, 2006·Journal of the American Chemical Society·Olga Crespo-BielJurriaan Huskens

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Citations

Apr 24, 2010·Analytical and Bioanalytical Chemistry·Marco FrasconiTommaso Ferri
Nov 20, 2012·Small·Mihaela GropeanuAránzazu del Campo
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jul 18, 2015·ACS Applied Materials & Interfaces·Claire SoumThomas Berthelot
Feb 23, 2013·International Journal of Molecular Sciences·Dorothee WasserbergPascal Jonkheijm
Apr 19, 2012·Chemistry : a European Journal·Dana A UhlenheuerLuc Brunsveld
Nov 26, 2008·Angewandte Chemie·Pascal JonkheijmHerbert Waldmann
Oct 17, 2009·Chemistry : a European Journal·Peter B CrowleyDmitri I Svergun
Jul 15, 2015·Bioinspiration & Biomimetics·S B OlssonT C Pearce
Oct 7, 2011·The Analyst·Colm T MallonTia E Keyes
Jul 6, 2014·Beilstein Journal of Organic Chemistry·Melanie RauschenbergBart Jan Ravoo
Jan 27, 2018·Chemical Communications : Chem Comm·D WasserbergP Jonkheijm
Feb 12, 2010·Angewandte Chemie·Benjamin W LeeSamuel K Sia
Nov 4, 2015·Chemistry : a European Journal·Ralph P G BosmansLuc Brunsveld
Jan 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Christian WendelnBart Jan Ravoo
Aug 19, 2009·Journal of the American Chemical Society·Shu-Han HsuJurriaan Huskens
Aug 26, 2014·Journal of the American Chemical Society·Jordi Cabanas-DanésPascal Jonkheijm
Oct 18, 2012·Analytical Chemistry·Kazuyo ShigetaJohn A Rogers
Aug 30, 2017·ACS Nano·Dorothee WasserbergPascal Jonkheijm

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