Ancient co-option of an amino acid ABC transporter locus in Pseudomonas syringae for host signal-dependent virulence gene regulation

PLoS Pathogens
Qing YanJeffrey C Anderson

Abstract

Pathogenic bacteria frequently acquire virulence traits via horizontal gene transfer, yet additional evolutionary innovations may be necessary to integrate newly acquired genes into existing regulatory pathways. The plant bacterial pathogen Pseudomonas syringae relies on a horizontally acquired type III secretion system (T3SS) to cause disease. T3SS-encoding genes are induced by plant-derived metabolites, yet how this regulation occurs, and how it evolved, is poorly understood. Here we report that the two-component system AauS-AauR and substrate-binding protein AatJ, proteins encoded by an acidic amino acid-transport (aat) and -utilization (aau) locus in P. syringae, directly regulate T3SS-encoding genes in response to host aspartate and glutamate signals. Mutants of P. syringae strain DC3000 lacking aauS, aauR or aatJ expressed lower levels of T3SS genes in response to aspartate and glutamate, and had decreased T3SS deployment and virulence during infection of Arabidopsis. We identified an AauR-binding motif (Rbm) upstream of genes encoding T3SS regulators HrpR and HrpS, and demonstrated that this Rbm is required for maximal T3SS deployment and virulence of DC3000. The Rbm upstream of hrpRS is conserved in all P. syringae stra...Continue Reading

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Citations

Dec 10, 2020·Computational and Structural Biotechnology Journal·Yingpeng XieXin Deng
Nov 24, 2020·Phytopathology·Yuan Zeng, Amy O Charkowski
Jul 3, 2021·Microorganisms·Megan R O'Malley, Jeffrey C Anderson

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Methods Mentioned

BETA
AlphaScreen
Illumina sequencing
PCR
genotyping
Protein Assay

Software Mentioned

BioPython
TREE
Excel
MAFFT
Inkscape
Minitab
BLASTP
BLAST
texshade
R

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