Androgen receptor-beta mRNA levels in different tissues in breeding and post-breeding male and female sticklebacks, Gasterosteus aculeatus

Reproductive Biology and Endocrinology : RB&E
Erik HoffmannBertil Borg

Abstract

Androgens induce male characters by activating androgen receptors (AR). Previous quantitative studies on AR in fishes have been limited to few tissues and/or a single season/reproductive state. The aim of this investigation was to study the possible role of AR-beta expression levels in the control of male traits in the three-spined stickleback. To that end, AR-beta expression levels in major tissues in breeding and post-breeding male and female sticklebacks were examined. AR-beta mRNA levels were quantified in ten tissues; eye, liver, axial muscle, heart, brain, intestine, ovary, testis, kidney and pectoral muscle in six breeding and post-breeding males and females using reverse transcription quantitative PCR. Breeding in contrast to post-breeding males built nests and showed secondary sexual characters (e.g. kidney hypertrophy) and elevated androgen levels. Post-breeding females had lower ovarian weights and testosterone levels than breeding females. AR-beta was expressed in all studied tissues in both sexes and reproductive states with the highest expression in the gonads and in the kidneys. The kidney is an androgen target organ in sticklebacks, from which breeding males produce the protein spiggin, which is used in nest-bui...Continue Reading

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T MiuraY Nagahama
Feb 20, 1999·The Journal of Biological Chemistry·J Takeo, S Yamashita
Mar 20, 1999·Biochimica Et Biophysica Acta·K TouhataS Yamashita
Aug 28, 1999·The Journal of Biological Chemistry·T IkeuchiY Nagahama
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masaaki AndoTomohiro Kozaka
May 10, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Hyuk Chu KwonJoon Yeong Kwon
Aug 19, 2005·Reproductive Biology and Endocrinology : RB&E·Per-Erik OlssonPeter Thomas
Jul 7, 2007·The Journal of Comparative Neurology·Lene K HarbottRussell D Fernald
Jul 14, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·C BjörkblomT Wiklund
Oct 19, 2007·Biology of Reproduction·Mohammad Sorowar HossainLaszlo Orban
Nov 26, 2008·General and Comparative Endocrinology·Xiaochun LiuHaoran Lin
Dec 20, 2008·BMC Evolutionary Biology·Véronique DouardYann Guiguen
Sep 16, 2010·The Journal of Biological Chemistry·Fang ZhouRongjia Zhou

Citations

Feb 1, 2013·Environmental Science & Technology·Johan SvenssonBjörn Brunström
Oct 16, 2015·Journal of Proteomics·Dietmar KültzEivind Almaas
Sep 17, 2013·Chemosphere·Anne Christine KnagIoanna Katsiadaki
Feb 21, 2020·Frontiers in Behavioral Neuroscience·Caitlin E LeslieHamilton E Farris

Related Concepts

Breeding
Kidney
Ovary
Androgen Receptor
Reproduction
Poly(A) Tail
Testis
Reverse Transcriptase Polymerase Chain Reaction
Gasterosteiformes
Androgens

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