Androgen receptors in the anterior pituitary and central nervous system of the androgen "insensitive" (Tfm) rat: correlation between receptor binding and effects of androgens on gonadotropin secretion

Endocrinology
O NaessF S French

Abstract

The cytosol fractions of the anterior pituitary, hypothalamus, preoptic area and brain cortex of androgen "insensitive" (Tfm) rats possess androgen receptors. However, in the Tfm rats the androgen binding per mg protein was only 10-15% of that in the corresponding normal littermates (Nl). The physicochemical properties of the androgen receptors in the anterior pituitary of the Tfm rat were indistinguishable from those of the normal rat. Thus, no distinctive differences were observed with regard to electrophoretic mobility in 3.25% polyacrylamide gels, isoelectric point (pI=5.8), binding affinity (KD=1.5 X 10(-9)M), temperature stability, sulfhydryl dependence and steroid specificity. It is, therefore, likely that the very low androgen binding capacity by the anterior pituitary and the central nervous system is due to an extreme reduction in the receptor number rather than to the presence of abnormal receptors. Since in the Tfm animals the androgen receptor number is reduced by 85-90%, it is to be expected that very high doses of androgens would be required to achieve hormonal effects. In fact, low doses of 5alpha-dihydrotestosterone propionate (50 mug/100 g body weight) given sc daily for 12 days had no effect on serum levels o...Continue Reading

Citations

Oct 20, 1978·European Journal of Applied Physiology and Occupational Physiology·A AakvaagF Fonnum
Nov 1, 1979·European Journal of Clinical Pharmacology·H FreyJ Falch
Jan 15, 1982·Experientia·K L Olsen, R E Whalen
Jun 9, 1987·Brain Research·M BonneauH H Feder
Jul 1, 1978·Journal of Steroid Biochemistry·O FridmanA F De Nicola
May 1, 1981·Journal of Steroid Biochemistry·S J Wieland, T O Fox
Jul 1, 1983·Journal of Steroid Biochemistry·T O FoxJ A Politch
Jan 1, 1993·Brain Research. Brain Research Reviews·S Segovia, A Guillamón
Dec 18, 1992·Brain Research. Developmental Brain Research·E M KurzD R Sengelaub
Jul 1, 1993·Brain Research. Molecular Brain Research·L H Burgess, R J Handa
Oct 1, 1977·Molecular and Cellular Endocrinology·K PurvisV Hansson
Jul 1, 1977·Molecular and Cellular Endocrinology·K PurvisV Hansson
Nov 1, 1982·Molecular and Cellular Endocrinology·F Kamel, L C Krey
Feb 17, 2005·Brain Research·Alicia Garcia-FalguerasAntonio Guillamon
Aug 29, 2000·Brain Research. Developmental Brain Research·K D Phelan, B W Newton
Jan 29, 2011·Clinical and Experimental Hypertension : CHE·Daniel ElyRolando Ramirez
Apr 28, 2009·Frontiers in Neuroendocrinology·Keith N FargoDale R Sengelaub
Apr 21, 2009·Physiology & Behavior·Dwayne K HamsonNeil V Watson
Jan 15, 2008·Hormones and Behavior·Dale R Sengelaub, Nancy G Forger
May 10, 2005·The Journal of Comparative Neurology·John A MorrisS Marc Breedlove
Apr 11, 2007·The European Journal of Neuroscience·Cristian Bodo, Emilie F Rissman
Mar 16, 1983·Biochemical and Biophysical Research Communications·K W ChungO M Rennert
Jul 1, 1984·American Journal of Physical Anthropology·O Lunde, P Grøttum

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