Androgen Suppresses Hyperosmolarity-Induced Inflammatory Mediators in Human Corneal Epithelial Cells.

Cornea
Noushin ZibandehAfsun Sahin

Abstract

To investigative the effects of sex steroids on hyperosmolar stress-induced proinflammatory cytokine expression of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-8, and IL-6, and on the mitogen-activated protein kinase pathway in immortalized human corneal epithelial cells (hCECs). Immortalized hCECs were cultured with keratinocyte-free medium until reaching 80% confluency with either 10 M dihidrotestosteron (DHT) or 10 M 17-β-estradiol, and then, the medium was changed to hyperosmolar for various time points. After hyperosmolar treatment, a real-time polymerase chain reaction was performed to show the TNF-α, IL-8, and IL-6 gene expression levels in hCECs. In addition, the treated cells were lysed, and Western blot analysis was applied for phosphorylated and nonphosphorylated forms of extracellular signal-regulated kinase 1/2 (ERK1/2), p38 kinase, and c-Jun N-terminal kinase 1/2 (JNK1/2). hCECs viability was measured with Annexin V/propidium iodide. Pretreatment with 10 M DHT or 17-β-estradiol inhibited the high osmolarity-induced expression of TNF-α, IL-8, and IL-6. The upregulation of p-ERK, p-JNK, and p-p38 with high osmolarity was inhibited partially by DHT, but 17-β-estradiol pretreatment only affected p-p38 for a s...Continue Reading

References

Dec 28, 1999·The British Journal of Ophthalmology·E M RochaD A Sullivan
Feb 9, 2000·American Journal of Physiology. Renal Physiology·L MicheaM B Burg
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·X LiG R Stark
Jul 31, 2003·American Journal of Ophthalmology·Debra A SchaumbergM Reza Dana
Jun 29, 2004·Progress in Retinal and Eye Research·Michael E Johnson, Paul J Murphy
Apr 14, 2005·The Journal of Clinical Endocrinology and Metabolism·S L DavisonS R Davis
May 26, 2006·Investigative Ophthalmology & Visual Science·Srihari NarayananAlison M McDermott
Sep 28, 2006·Investigative Ophthalmology & Visual Science·Alan TomlinsonAngus McFadyen
Jul 16, 2010·Investigative Ophthalmology & Visual Science·Benjamin D SullivanMichael A Lemp
Dec 6, 2014·Progress in Retinal and Eye Research·R J BraunN R Gewecke
Feb 3, 2016·Investigative Ophthalmology & Visual Science·Anna F AblamowiczKelly K Nichols
Apr 15, 2016·The British Journal of Ophthalmology·Blanka GolebiowskiFiona Stapleton
Oct 22, 2016·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Jay S MishraSathish Kumar
Jul 25, 2017·The Ocular Surface·Jennifer P CraigFiona Stapleton
Mar 20, 2018·Molecular Metabolism·Katya B Rubinow
May 15, 2018·Frontiers in Immunology·Melanie R Gubbels Bupp, Trine N Jorgensen
Dec 26, 2018·Journal of the Endocrine Society·Vittorio Emanuele Bianchi

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