Androgens upregulate aquaporin 9 expression in the prostate

International Journal of Urology : Official Journal of the Japanese Urological Association
Jianbo WangMasayoshi Yokoyama

Abstract

Aquaporins (AQP) function as selective pores allowing water, glycerol and other small solutes to pass through the cell membrane. We previously reported that AQP are expressed in the prostates of both humans and rats. The present study investigated the androgen-dependent expression of AQP9 in the prostate in vivo and in vitro. Rat ventral prostate tissue specimens and a normal human prostatic epithelial cell line (PNT2) were used. AQP9 messenger ribonucleic acid (mRNA) and protein expression were examined using real-time polymerase chain reaction, Western blotting and immunohistochemical methods. Androgen modulation was achieved by surgical castration, treatment with testosterone propionate (5 microg/g bodyweight) or bicalutamide (20 microg/g bodyweight), and the ribonucleic acid interference (RNAi) method of the androgen receptor (AR). The synthetic small interfering RNA was transfected into PNT2 at a concentration of 10 nM, and the RNAi effect was evaluated using a Western blotting analysis. AQP9 mRNA and protein were expressed in the rat prostate. Surgical castration or bicalutamide treatment significantly decreased their expression. In addition, the treatment with testosterone propionate after castration restored the express...Continue Reading

References

Feb 1, 1978·The Journal of Physiology·P Y Wong, C H Yeung
Jan 1, 1983·Australian Journal of Biological Sciences·D E Brooks
Jan 1, 1995·Methods in Cell Biology·M C Colombel, R Buttyan
Oct 1, 1996·The Journal of Membrane Biology·J H Park, M H Saier
Sep 12, 1998·The Journal of Biological Chemistry·H TsukaguchiM A Hediger
Jul 24, 2001·Biology of Reproduction·N Pastor-SolerD Brown
Nov 29, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G P NicchiaM Svelto
May 22, 2002·Biology of Reproduction·Nuria Pastor-SolerSylvie Breton
Jan 11, 2003·Calcified Tissue International·W WangT C Hart
Apr 6, 2005·Drug Discovery Today·Neil A Castle
May 3, 2006·BMC Biochemistry·Daniel A GorelickPeter Agre
Nov 27, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Jianbo WangMasayoshi Yokoyama

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Citations

Aug 29, 2013·The Journal of Sexual Medicine·Lijun PeiXilun Gan
Feb 22, 2012·Molecular Aspects of Medicine·Dan ZhangHe-Feng Huang
Apr 13, 2010·Neurochemistry International·Marina Zelenina
Mar 20, 2013·Reproduction in Domestic Animals = Zuchthygiene·M GrzesiakM R Luck
May 18, 2016·International Journal of Molecular Sciences·Qiwei ChenJianbo Wang
Nov 7, 2016·Pathology Oncology Research : POR·Ji Y Park, GhilSuk Yoon
Mar 31, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Cecilia LindskogMichael Rützler

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