Andrographis paniculata transcriptome provides molecular insights into tissue-specific accumulation of medicinal diterpenes

BMC Genomics
Anchal GargSumit Ghosh

Abstract

Kalmegh (Andrographis paniculata) has been widely exploited in traditional medicine for the treatment of infectious diseases and health disorders. Ent-labdane-related diterpene (ent-LRD) specialized (i.e., secondary) metabolites of kalmegh such as andrographolide, neoandrographolide and 14-deoxy-11,12-didehydroandrographolide, are known for variety of pharmacological activities. However, due to the lack of genomic and transcriptomic information, underlying molecular basis of ent-LRDs biosynthesis has remained largely unknown. To identify candidate genes of the ent-LRD biosynthetic pathway, we performed comparative transcriptome analysis using leaf and root tissues that differentially accumulate ent-LRDs. De novo assembly of Illumina HiSeq2000 platform-generated paired-end sequencing reads resulted into 69,011 leaf and 64,244 root transcripts which were assembled into a total of 84,628 unique transcripts. Annotation of these transcripts to the Uniprot, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Carbohydrate-Active Enzymes (CAZy) databases identified candidate transcripts of the ent-LRD biosynthetic pathway. These included transcripts that encode enzymes of the plastidial 2C-methyl-D-erythritol-4-phosphate pathway which p...Continue Reading

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Citations

Sep 2, 2016·Frontiers in Plant Science·Neeraja CherukupalliDashavantha R Vudem
Dec 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Megha RaiMami Yamazaki
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Sep 28, 2021·Applied Microbiology and Biotechnology·Hosakatte Niranjana Murthy, Dayanand Dalawai

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Datasets Mentioned

BETA
SRP044357

Methods Mentioned

BETA
PCR
Chip
glycosylation
MDS
genotyping

Software Mentioned

Oases
ncbi
Bowtie
MISA
SeqQC
Bowtie tool
Predotar
DegSeq
MIcroSAtellite
BLAST

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