Andrographolide Exhibits Anticancer Potential Against Human Colon Cancer Cells by Inducing Cell Cycle Arrest and Programmed Cell Death via Augmentation of Intracellular Reactive Oxygen Species Level

Nutrition and Cancer
Imran KhanIrfan A Ansari

Abstract

Andrographolide, a diterpenoid lactone and a major constituent of Andrographis paniculata Nees, exhibits remarkable anticancer activity. However, the effect of andrographolide on colon cancer has not been completely elucidated yet. Thus, we investigated the chemopreventive potential of andrographolide in colon cancer HT-29 cells. The cytotoxic potential of andrographolide on HT-29 cells was determined by MTT assay, trypan blue exclusion assay, colony formation assay, and morphological analysis; and apoptotic property by DAPI and Hoechst staining, FITC-Annexin V assay, DNA fragmentation assay and caspase-3 activity assay. To elucidate andrographolide action, intracellular reactive oxygen species (ROS) level was determined by DCFDA dye; change in mitochondrial potential by Rhodamine123 and Mito Tracker Red CMXRos dye; and cell cycle modulatory property by flow cytometric analysis. Results of the study have shown that andrographolide decreased cell viability of HT-29 cells in a dose- and time-dependent manner. Furthermore, andrographolide induced apoptosis in HT-29 cells which seemed to be linked with augmented intracellular ROS level and disruption of mitochondrial membrane potential. Interestingly, andrographolide caused signifi...Continue Reading

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Citations

Nov 24, 2019·Antioxidants·Eugenie MussardHechmi Toumi
Nov 28, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Imran KhanIrfan A Ansari
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thitianan KulsiriratKorbtham Sathirakul
Jul 30, 2021·Frontiers in Cardiovascular Medicine·Mao LiuYin Huang
Dec 9, 2020·Journal of Medicinal Chemistry·Yulin Ren, A Douglas Kinghorn
Jul 18, 2020·Anti-cancer Agents in Medicinal Chemistry·Imran KhanIrfan A Ansari

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Methods Mentioned

BETA
Assay
electrophoresis
FACS
fluorescence microscopy
flow cytometry
phase contrast microscopy

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