PMID: 8594318Jan 1, 1996Paper

Androstenedione increases thromboxane A2 receptors in human erythroleukemia cells

Life Sciences
T P ZuckerP V Halushka

Abstract

Previous studies have demonstrated an increased thromboxane A2 (TXA2) receptor expression in human erythroleukemia (HEL) cells and rat aortic smooth muscle (RASM) cells in response to testosterone treatment. HEL cells have served as a model for megakaryocytes, the progenitor cell for platelets. Platelets have previously been shown to convert androstenedione to testosterone. This study investigated the effects of androstenedione on the TXA2 receptor density in HEL and cultured RASM cells. Both cell lines were incubated with vehicle, 150 nM testosterone or 250, 500 or 750nM androstenedione for 48 hours. Co-incubation with testosterone or androstenedione significantly (p<0.05) increased the maximum number of TXA2 binding sites (Bmax) in HEL cells compared to controls. There was no significant change in Kd values. In a separate series of experiments, HEL cells were incubated with the androgen receptor antagonist hydroxyflutamide (2.5mM). Treatment with androstenedione (500nM) significantly (p<0.05) increased the Bmax value by 35% compared to control and hydroxyflutamide completely antagonized this effect of androstenedione. Incubation with hydroxyflutamide alone had no effect on the Bmax values compared to control. RASM cells also ...Continue Reading

References

Oct 1, 1976·Annals of Internal Medicine·W B KannelT Gordon
Jun 1, 1990·Maturitas·A SchlemmerC Christiansen
Jul 11, 1989·Biochimica Et Biophysica Acta·D E MaisP V Halushka
Jan 1, 1989·Annual Review of Pharmacology and Toxicology·P V HalushkaT A Morinelli
Jun 30, 1988·The New England Journal of Medicine·K T KhawE Barrett-Connor
Oct 16, 1986·The New England Journal of Medicine·D J FitzgeraldG A FitzGerald
Apr 30, 1987·The New England Journal of Medicine·G A ColditzC H Hennekens
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jan 1, 1984·Annual Review of Public Health·D L Wingard
May 1, 1982·The Journal of Clinical Endocrinology and Metabolism·L Milewich, M G Whisenant
May 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·J C PenhosP Ramwell
Feb 1, 1994·European Journal of Clinical Investigation·K SchrörP V Halushka
Jan 1, 1993·Annual Review of Pharmacology and Toxicology·R W Rockhold
May 1, 1991·Trends in Cardiovascular Medicine·T A Morinelli, P V Halushka

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Citations

Mar 6, 2008·Kidney & Blood Pressure Research·Tina Kienitz, Marcus Quinkler
Feb 26, 2015·Journal of Thrombosis and Haemostasis : JTH·K JimenezC Gasche
Oct 25, 2002·Cardiovascular Drug Reviews·Wolfgang Weidemann, Hartmut Hanke
May 4, 2020·Steroids·Krishika Sambyal, Rahul Vikram Singh

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