Anesthetic actions of thiopental remain largely unaffected during cholinergic overstimulation in cultured cortical networks

Toxicology Letters
Isabel WeimerMonika Balk

Abstract

In case of military or terrorist use of organophosphorus (OP) compounds victims are likely to suffer from not only intoxication but physical trauma as well. Appropriate emergency care may therefore include general anesthesia to allow life-saving surgical intervention. Since there is evidence that drug potency and efficacy of several anesthetics are attenuated by high concentrations of acetylcholine in the CNS, this study was designed to evaluate the anesthetic actions of thiopental during cholinergic overstimulation. Making use of organotypic slice cultures derived from the mouse neocortex, drug effects were assessed by extracellular voltage recordings of network activity at basal cholinergic tone and during simulated cholinergic crisis (high cholinergic tone). The latter was achieved by inhibition of acetylcholinesterases via soman and an ambient acetylcholine concentration of 10μM. The induction of cholinergic crisis in vitro increased the network activity of cortical neurons significantly. Surprisingly, differences in network activity between basal and high cholinergic tone became less pronounced with rising concentrations of thiopental and drug potency and efficacy were almost equivalent. These results clearly distinguish t...Continue Reading

References

Dec 1, 1981·Journal of Neuroscience Methods·B H Gähwiler
Feb 17, 1994·Nature·N P Franks, W R Lieb
Apr 1, 1994·Neurochemistry International·T HashimotoK Kuriyama
Feb 1, 1996·Anesthesiology·L L GustafssonD R Stanski
Dec 17, 1997·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·A FassoulakiC Derveniotis
Sep 18, 1998·Clinical Pharmacokinetics·H Russo, F Bressolle
Apr 20, 2002·Anesthesiology·Robert DickinsonNicholas P Franks
Aug 14, 2002·British Journal of Anaesthesia·B W Urban
Aug 14, 2002·British Journal of Anaesthesia·J P Dilger
Oct 3, 2002·Anesthesiology·Ron Ben AbrahamUNKNOWN Department of Anesthesiology and Critical Care Medicine, Tel Aviv Sourasky Medical Center and the Sackler Faculty of Medicin
Oct 16, 2002·British Journal of Anaesthesia·S M White
Feb 25, 2003·Anesthesia and Analgesia·Jukka Takala, Rudolph H de Jong
Aug 24, 2004·Nature Reviews. Neuroscience·Uwe Rudolph, Bernd Antkowiak
Mar 15, 2006·Current Opinion in Anaesthesiology·Christian GrasshoffBernd Antkowiak
May 26, 2007·Anesthesiology·Christian GrasshoffBernd Antkowiak
Apr 22, 2008·Nature Reviews. Neuroscience·Nicholas P Franks
Aug 11, 2010·Toxicology Letters·Berthold DrexlerChristian Grasshoff
Nov 16, 2011·The European Journal of Neuroscience·Harald Hentschke, Maik C Stüttgen
Apr 23, 2013·Pharmacology & Therapeutics·Franz Worek, Horst Thiermann
Oct 5, 2013·Chemico-biological Interactions·Horst ThiermannKai Kehe

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Citations

Oct 12, 2018·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Katharina MarquartTimo Wille

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