Anesthetics modulate phospholipase C hydrolysis of monolayer phospholipids by surface pressure

Chemistry and Physics of Lipids
D M GoodmanP M Winter

Abstract

Anesthetics are believed to produce anesthesia through the reversible inhibition of synaptic transmission but how this is accomplished is unknown. Based on earlier studies of anesthetic-enzyme-phospholipid interaction, we surmised that anesthetics may inhibit synaptic transmission by increasing synaptic membrane lateral pressure thereby inhibiting phospholipid hydrolysis, membrane transduction and synaptic transmission. As a first approximation towards investigating this concept, we hypothesized that anesthetics modulate the rate of phospholipase C hydrolysis of a lipid monolayer through its effects on surface pressure. The relationship between the hydrolysis rate of a monolayer of dipalmitoylphosphatidylcholine [14C-choline] (DPPC) by phospholipase C (Plase C) and monolayer surface pressure (SP) as altered by either halothane, isoflurane, or by physical compression at 37 degrees C was studied. The decline in surface 14C-activity as the [14C]choline diffuses into the Krebs-Ringer bicarbonate buffer aqueous subphase is estimated as the rate of DPPC hydrolysis measured by the initial slope method. DPPC hydrolysis was about 300 cpm/min and constant between SP of 0 to 20 dynes/cm. Higher SP between 25 and 30 dyne/cm, whether induce...Continue Reading

References

Jan 1, 1979·Annual Review of Pharmacology and Toxicology·S H Roth
Oct 1, 1978·Chemistry and Physics of Lipids·R W Evans, J Tinoco
Jul 1, 1976·Chemistry and Physics of Lipids·R Verger, F Pattus
Jan 1, 1987·Annual Review of Physiology·B Deuticke, C W Haest
Apr 22, 1988·Biochimica Et Biophysica Acta·B López-CorcueraC Aragón
Sep 21, 1987·Life Sciences·P E Keane, K Biziere
Jan 1, 1986·The Journal of Membrane Biology·M C Sekar, L E Hokin
Oct 1, 1987·Chemistry and Physics of Lipids·S NuryR Verger
Sep 25, 1986·Biochimica Et Biophysica Acta·W D Sweet, F Schroeder
Jan 1, 1986·Anesthesiology·I UedaH Kamaya
Sep 1, 1973·The Journal of Physiology·C D Richards
Apr 1, 1972·Anesthesiology·K W MillerR A Smith
Oct 1, 1984·Anesthesia and Analgesia·I Ueda, H Kamaya
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D E GreenG A Blondin
May 1, 1980·Quarterly Reviews of Biophysics·J N IsraelachviliR G Horn
Feb 17, 1994·Nature·N P Franks, W R Lieb
Jul 1, 1993·Nature·S J SlaterC D Stubbs
Aug 1, 1963·International Anesthesiology Clinics·J A CLEMENTS, K M WILSON
Mar 1, 1952·Journal of Neurophysiology·M G LARRABEE, J M POSTERNAK

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Citations

Mar 28, 2008·European Biophysics Journal : EBJ·M Weis, M Kopáni
Nov 12, 2014·The Journal of General Physiology·Karl F HeroldHugh C Hemmings
Jul 5, 2001·Colloids and Surfaces. B, Biointerfaces·A ShibataT Higuti

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