Aneuploidy and Ethanol Tolerance in Saccharomyces cerevisiae

Frontiers in Genetics
Miguel MorardEladio Barrio

Abstract

Response to environmental stresses is a key factor for microbial organism growth. One of the major stresses for yeasts in fermentative environments is ethanol. Saccharomyces cerevisiae is the most tolerant species in its genus, but intraspecific ethanol-tolerance variation exists. Although, much effort has been done in the last years to discover evolutionary paths to improve ethanol tolerance, this phenotype is still hardly understood. Here, we selected five strains with different ethanol tolerances, and used comparative genomics to determine the main factors that can explain these phenotypic differences. Surprisingly, the main genomic feature, shared only by the highest ethanol-tolerant strains, was a polysomic chromosome III. Transcriptomic data point out that chromosome III is important for the ethanol stress response, and this aneuploidy can be an advantage to respond rapidly to ethanol stress. We found that chromosome III copy numbers also explain differences in other strains. We show that removing the extra chromosome III copy in an ethanol-tolerant strain, returning to euploidy, strongly compromises its tolerance. Chromosome III aneuploidy appears frequently in ethanol-tolerance evolution experiments, and here, we show t...Continue Reading

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Citations

Jun 15, 2019·Yeast·Ciaran Gilchrist, Rike Stelkens
Aug 18, 2020·AMB Express·Li ShenDao-Qiong Zheng
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Feb 8, 2021·International Journal of Food Microbiology·Estéfani García-RíosJosé Manuel Guillamón
Mar 28, 2021·Applied and Environmental Microbiology·M Lairón-PerisA Querol

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Datasets Mentioned

BETA
ERS484054
GSE44863
PRJNA493718

Methods Mentioned

BETA
flow cytometry
PCR
electrophoresis
protein folding

Software Mentioned

Augustus
MAFFT
Sickle
gdools
MUMmer
Velvet
breseq
RAxML
SSPACE
Advanced Relative Quantification

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