PMID: 7537466Apr 1, 1995Paper

ANF and angiotensin II interact via kinases in the proximal straight tubule

The American Journal of Physiology
N H Garcia, J L Garvin

Abstract

Atrial natriuretic factor (ANF) inhibits fluid absorption (Jv) in the proximal straight tubule (PST) only after stimulation with angiotensin II (ANG II). To investigate ANF's dependency on ANG II for transport inhibition, we blocked and mimicked angiotensin's second messenger cascades and then examined ANF's ability to inhibit Jv. ANG II (10(-10) M)-stimulated Jv was 0.47 +/- 0.10 nl.mm-1. min-1. After ANF (10(-10) M) was added to the bath, Jv fell by approximately 40% (P < 0.05). ANG II stimulates Jv via activation of protein kinase C (PKC) and decreasing protein kinase A (PKA) activity. We inhibited PKA with H-89. In the presence of only H-89, Jv was 0.75 +/- 0.11 nl.mm-1.min-1. After ANF was added to the bath Jv fell by 30% (P < 0.05). Intracellular adenosine 3',5'-cyclic monophosphate content was not affected by ANF in the presence of ANG II. ANF could not inhibit Jv in the presence of ANG II and 3-isobutyl-1-methylxanthine, a phosphodiesterase inhibitor. KT-5823, a guanosine 3',5'-cyclic monophosphate (cGMP)-dependent protein kinase inhibitor, blocked the action of ANF on Jv (P > 0.30). PKC inhibition did not prevent the decrease in Jv induced by ANF. We conclude that ANF inhibits ANG II-induced stimulation of transport by...Continue Reading

Citations

Jun 16, 2001·American Journal of Physiology. Renal Physiology·T HannkenG Wolf
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Kazuyoshi NakamuraManabu Kubokawa
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D M Boesch, J L Garvin
Oct 27, 2007·In Vitro Cellular & Developmental Biology. Animal·Philip G WoostUlrich Hopfer

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