Ang-(1-7) protects HUVECs from high glucose-induced injury and inflammation via inhibition of the JAK2/STAT3 pathway

International Journal of Molecular Medicine
Jianfang ChenJingfu Chen

Abstract

Angiotensin (Ang)‑1‑7, which is catalyzed by angiotensin‑converting enzyme 2 (ACE2) from angiotensin‑II (Ang‑II), exerts multiple biological and pharmacological effects, including cardioprotective effects and endothelial protection. The Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway has been demonstrated to be involved in diabetes‑associated cardiovascular complications. The present study hypothesized that Ang‑(1‑7) protects against high glucose (HG)‑induced endothelial cell injury and inflammation by inhibiting the JAK2/STAT3 pathway in human umbilical vein endothelial cells (HUVECs). HUVECs were treated with 40 mmol/l glucose (HG) for 24 h to establish a model of HG‑induced endothelial cell injury and inflammation. Protein expression levels of p‑JAK2, t‑JAK2, p‑STAT3, t‑STAT3, NOX‑4, eNOS and cleaved caspase‑3 were tested by western blotting. CCK‑8 assay was performed to assess cell viability of HUVECs. Apoptotic cell death was analyzed by Hoechst 33258 staining. Mitochondrial membrane potential (MMP) was obtained using JC‑1. Superoxide dismutase (SOD) activity was tested by SOD assay kit. Interleukin (IL)‑1β, IL‑10, IL‑12 and TNF‑α levels in culture media were tested by ELISA. The fi...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 21, 2004·American Journal of Physiology. Cell Physiology·Chih-Wen NiDanny Ling Wang
Jan 11, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Ibrahim F BenterDebra I Diz
Nov 27, 2008·Kidney International·Heather N ReichAndrew M Herzenberg
Nov 10, 2009·Diabetes Research and Clinical Practice·J E ShawP Z Zimmet
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tijana CopfThomas Preat
May 19, 2011·Cancer Research·Ryan B CorcoranNabeel Bardeesy
Feb 22, 2012·Growth Factors·Hiu Kiu, Sandra E Nicholson
May 15, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mariam H M YousifIbrahim F Benter
Oct 4, 2012·Clinical and Experimental Pharmacology & Physiology·Hui-Yu YangZhi-Ming Yang
Dec 13, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Lijun WangFang Tian
Oct 30, 2013·Basic & Clinical Pharmacology & Toxicology·Saara Aittomäki, Marko Pesu
Mar 7, 2014·Clinical Science·Clare A McKinneyStuart A Nicklin
Mar 19, 2014·Diabetes Research and Clinical Practice·L GuariguataJ E Shaw
Aug 30, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiao-Mei WangLiang-Yi Si
Oct 9, 2014·Pflügers Archiv : European journal of physiology·Carla MenesesClaudio Cabello-Verrugio
Feb 28, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yue-Hui ZhangYun Zhang
Mar 1, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Pan-Pan HaoYun Zhang
Apr 22, 2015·Apoptosis : an International Journal on Programmed Cell Death·Xue JiangFeng-he Du
Jun 16, 2015·British Journal of Pharmacology·A M PapinskaK E Rodgers
Aug 19, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Daniela SuricoElena Grossini
Sep 26, 2015·Biochemical and Biophysical Research Communications·Yuedong MaYili Chen
Dec 8, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Indiwari Gopallawa, Bruce D Uhal
Jan 23, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Rosa Bretón-RomeroNaomi M Hamburg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cardiovascular Disorder in Diabetes

Diabetes is associated with an increased risk of cardiovascular disorders and heart failure. Discover the latest research here.