ANG II and vasopressin stimulate calcium entry in dispersed smooth muscle cells of preglomerular arterioles

The American Journal of Physiology
Bjarne M Iversen, William J Arendshorst

Abstract

Calcium signaling mechanisms were examined in vessel segments and dispersed single smooth muscle cells (SMC) of interlobular arteries and afferent arterioles (< 50 microns diameter) from the rat kidney. These resistance vessels were isolated from rat kidneys, using an iron oxide-sieving technique with subsequent collagenase digestion. Individual cells were identified by their characteristic oval appearance and positive staining for smooth muscle-specific alpha-actin and heavy chain myosin SM-1 and SM-2. Cytosolic calcium concentration ([Ca2+]i) was measured using fura 2 ratiometric fluorescence at 340 and 380 nm wavelength with a microscope-based photometer. Angiotensin II (ANG II) and arginine vasopressin (AVP), at concentrations of 10(-10)-10(-6) M, produced dose-dependent increases in [Ca2+]i; maximum increases were 221 +/- 49 nM for ANG II and 237 +/- 49 nM for AVP. The temporal response patterns for both agonists were characterized by a square-shaped, immediate step increase in [Ca2+]i to a near maximum level that was maintained through the recording period of 150-200 s. Responses of individual dispersed SMC and short vessel segments were similar. Losartan antagonized the action of ANG II, indicating mediation by AT1 recep...Continue Reading

References

Mar 5, 1992·Regulatory Peptides·M Thibonnier
Apr 1, 1992·Pflügers Archiv : European journal of physiology·P E JensenC Aalkjaer
Nov 13, 1991·European Journal of Pharmacology·C MironneauJ Mironneau
Nov 11, 1990·Cell Calcium·J W Putney
Aug 1, 1990·FEBS Letters·T J Rink
Oct 14, 1987·Biochemical and Biophysical Research Communications·A ZschauerC van Breemen
Jul 1, 1995·Physiological Reviews·G K Owens
Jan 1, 1995·Naunyn-Schmiedeberg's Archives of Pharmacology·V BuryiT Godfraind
Aug 1, 1994·Canadian Journal of Physiology and Pharmacology·E L Schiffrin
Jan 1, 1994·Annual Review of Physiology·J G Douglas, U Hopfer
Apr 1, 1996·Physiological Reviews·L G NavarK D Mitchell
Jan 1, 1996·Cellular Signalling·M B MarreroK E Bernstein

❮ Previous
Next ❯

Citations

Dec 18, 2003·American Journal of Physiology. Renal Physiology·Carie S FacemireWilliam J Arendshorst
Nov 13, 2004·American Journal of Physiology. Renal Physiology·Rachel W FalletPamela K Carmines
Dec 16, 2004·American Journal of Physiology. Renal Physiology·Frank H HansenBjarne M Iversen
Jun 9, 2005·American Journal of Physiology. Renal Physiology·Andrew J FullerL Gabriel Navar
Sep 27, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher S Wilcox
Jun 3, 2000·American Journal of Physiology. Renal Physiology·O VågnesW J Arendshorst
Sep 25, 2003·American Journal of Physiology. Renal Physiology·Joen SteendahlMax Salomonsson
Apr 13, 2004·American Journal of Physiology. Renal Physiology·Max Salomonsson, William J Arendshorst
Jan 1, 2009·American Journal of Physiology. Renal Physiology·Donald J MarshNiels-Henrik Holstein-Rathlou
Mar 21, 2018·Current Hypertension Reports·Ewa Szczepanska-SadowskaAgnieszka Cudnoch-Jedrzejewska
Jul 18, 2001·American Journal of Physiology. Renal Physiology·M Salomonsson, W J Arendshorst
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Max SalomonssonNiels-Henrik Holstein-Rathlou
Sep 16, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Linda MagnussonMax Salomonsson
Jan 11, 2008·American Journal of Physiology. Renal Physiology·Qingli ZhangThomas L Pallone
Mar 14, 2014·American Journal of Physiology. Renal Physiology·Nicholas G MossWilliam J Arendshorst
Jan 20, 2005·American Journal of Physiology. Renal Physiology·Oyvind B VagnesWilliam J Arendshorst
Jan 13, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Charlotte Mehlin SorensenNiels-Henrik Holstein-Rathlou
May 15, 2009·American Journal of Physiology. Renal Physiology·Tiffany L ThaiWilliam J Arendshorst
Jul 16, 2010·American Journal of Physiology. Renal Physiology·Zhong ZhangThomas L Pallone
Feb 10, 2006·American Journal of Physiology. Renal Physiology·Frank HelleBjarne M Iversen
Jun 13, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sofia JönssonMichael Hultström
Jan 9, 2009·American Journal of Physiology. Renal Physiology·Edward W InschoLi-Ming Jin
May 25, 2002·Kidney International·Susan K Fellner, William J Arendshorst

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of the American Society of Nephrology : JASN
Bjarne M Iversen, William J Arendshorst
The American Journal of Physiology
S K Fellner, William J Arendshorst
© 2022 Meta ULC. All rights reserved