Angelica sinensis polysaccharide inhibits proliferation, migration, and invasion by downregulating microRNA-675 in human neuroblastoma cell line SH-SY5Y

Cell Biology International
Jing YangLirong Sun

Abstract

Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the underlying mechanisms. Effects of AP on cell viability, proliferation, apoptosis, migration, invasion, and expressions of long noncoding RNA H19 (lncRNA-H19), microRNA (miR)-675, and CD44 were assessed. Then, effects of miR-675 overexpression on AP-treated cells were analyzed. Next, expression of key kinases in the PI3K/AKT and JAK/ STAT pathways was detected. The possible target gene of miR-675 was finally explored. Cell viability was reduced by 200-500 µg/mL AP. Meanwhile, AP repressed cell proliferation, migration, and invasion, but induced apoptosis. Expressions of lncRNA-H19 and miR-675 were upregulated in neuroblastoma cells, and were downregulated by AP. AP was also identified to upregulate CD44. We next found AP affected SH-SY5Y cells through downregulating miR-675. Key kinases in the PI3K/AKT and JAK/STAT pathways were downregulated by AP stimulation, while these downregulations were abrogated by miR-675 overex...Continue Reading

References

Jan 1, 1996·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·V CombaretM C Favrot
Jun 17, 1998·Genes & Development·V Cryns, J Yuan
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·David E Levy, J E Darnell
Dec 11, 2002·Current Opinion in Cell Biology·Jerry M Adams, Suzanne Cory
Sep 3, 2009·Annals of the New York Academy of Sciences·Bharat B AggarwalBokyung Sung
Jul 19, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Jun QinLiao-Bin Chen
Jan 1, 2014·Cancer Letters·Jin Ding, Hongyang Wang
Jan 7, 2014·Biochemical and Biophysical Research Communications·Ming ZhuangYongqian Shu
Jul 22, 2014·International Journal of Cancer. Journal International Du Cancer·Kristina AlthoffJohannes H Schulte
Nov 12, 2014·Annual Review of Medicine·Chrystal U Louis, Jason M Shohet
Jul 8, 2015·Biochimica Et Biophysica Acta·Julia Liz, Manel Esteller
Aug 28, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jue ZhangMengliang Tian
Oct 4, 2015·Biochemical and Biophysical Research Communications·Wei-Jie ZhouCai-Juan Song
Oct 17, 2015·Annual Review of Medicine·Ingrid A Mayer, Carlos L Arteaga
Jan 8, 2016·International Immunopharmacology·Kaiping WangYu Zhang
Feb 18, 2016·Oncotarget·Marco FidaleoMaria Paola Paronetto
Feb 26, 2016·Journal of Natural Medicines·Chunmei WangJianguang Chen
Mar 15, 2016·Current Diabetes Reports·Kate SimpsonTimothy Bowen
Apr 9, 2016·Oncoimmunology·Carmela De Santo, Francis Mussai
Mar 23, 2017·Frontiers in Cell and Developmental Biology·Linda T Senbanjo, Meenakshi A Chellaiah
Mar 30, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Beibei BieZongfang Li
Apr 1, 2017·Singapore Medical Journal·Foon Yin Fung, Yeh Ching Linn
Jul 26, 2017·Nature Genetics·Valentina BoevaIsabelle Janoueix-Lerosey
Sep 10, 2017·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wen-Feng ZhangMing-Hua Duan

❮ Previous
Next ❯

Citations

Jun 19, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Yongzhen XueHui Li
Jun 8, 2019·Molecular Medicine Reports·Qian-Kun JiBao-Zhe Jin
Jun 17, 2020·Frontiers in Pharmacology·Qing-Xia GanQin-Wan Huang
Jul 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min-Zhe WangTie-Hong Yang
Jun 7, 2021·International Journal of Biological Macromolecules·Jijuan NaiHuagang Sheng
Jul 1, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hui Pan, Linlin Zhu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.