PMID: 7543630Aug 1, 1995Paper

Angiogenesis in a delayed revascularization model is accelerated by angiogenic oligosaccharides of hyaluronan

Laboratory Investigation; a Journal of Technical Methods and Pathology
V C LeesD C West

Abstract

A delayed revascularisation model has been used to assess the angiogenic activity of hyaluronan fragments on impaired wound healing. Revascularisation of single, full thickness skin autografts in rats was delayed by subjecting isolated grafts to a sublethal cryoinjury (freeze injury) before implantation. Hyaluronan fragments were delivered to the grafts using slow release pellets (ethylene vinyl acetate copolymer). Rates of release were measured in vitro by ELISA. The angiogenic response to the application of 100 micrograms of low (1 to 4 kDa) molecular weight hyaluronan and 100 micrograms of medium (33 kDa) molecular weight hyaluronan was tested in separate groups of 15 rats and was compared with unstimulated cryoinjured controls (n = 40). The effect of low (1 to 4 kDa) molecular weight hyaluronan on uninjured grafts was also investigated. Return of graft blood flow was measured on anesthetised animals over a 10-day period using laser Doppler flowmetry and 133Xe clearance. Quantitative histologic assessment of the graft vasculature was performed on Days 3, 7, and 10 after implantation. The 1- to 4-kDa hyaluronan fragments increased blood flow (p < 0.001), as measured by both flow measuring techniques, and increased graft vesse...Continue Reading

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