Angiogenesis in Balb/c mice under beta-carotene supplementation in diet.

Genes & Nutrition
Urszula RaznyAldona Dembinska-Kiec

Abstract

Angiogenesis is a process of new blood vessel formation from pre-existing ones. The most important steps in angiogenesis include detachment, proliferation, migration, homing and differentiation of vascular wall cells, which are mainly endothelial cells and their progenitors. The study focused on the effect of beta-carotene (BC) supplementation (12,000 mg/kg) in the diet on angiogenesis in Balb/c mice. Female Balb/c mice were fed for 5 weeks with two different diets: with BC or without BC supplementation. After 4 weeks of feeding, Balb/c mice were injected subcutaneously with two matrigel plugs with or without basic fibroblast growth factor (bFGF). Six days later, the animals were killed, and the matrigel plugs were used for immunohistochemical staining with CD31 antibody and for gene expression analysis. Microarray and Real-Time PCR data showed down-regulation of genes involved in proliferation and up-regulation of genes encoding inhibitors of apoptosis, proteins regulating cell adhesion, matrix-degrading enzymes and proteins involved in the VEGF pathway. The results of this study demonstrated that BC proangiogenic activity (with or without bFGF) in vivo seemed to be more significantly associated with cells' protection from apo...Continue Reading

References

Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·S KamadaY Tsujimoto
Oct 6, 1999·Current Opinion in Cell Biology·G BazzoniM G Lampugnani
Nov 26, 1999·The Journal of Nutrition·C M LeeJ W Erdman
Jul 14, 2000·Physiological Reviews·S A RossL M De Luca
Apr 10, 2002·American Journal of Physiology. Cell Physiology·Michael Papetti, Ira M Herman
Feb 18, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Geerten P van Nieuw AmerongenVictor W M van Hinsbergh
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Feb 27, 2004·Biochimica Et Biophysica Acta·Onno KranenburgEmile E Voest
Mar 24, 2004·Critical Reviews in Oncology/hematology·Georges BellonArnaud Robinet
Nov 26, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Bradley K McCollMarc G Achen
Dec 17, 2004·Journal of Cellular and Molecular Medicine·Holger RumpoldEberhard Gunsilius
May 19, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Beata Kieć-WilkAldona Dembińska-Kieć
Jun 14, 2005·Biochimica Et Biophysica Acta·A Dembinska-KiecG Schmitz
Feb 20, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·E A van BeekJ Keijer

❮ Previous
Next ❯

Citations

Apr 4, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huei-Yan ChenMiao-Lin Hu
Jun 29, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Colleen G Le PrellKevin K Ohlemiller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.