Angiogenesis PET Tracer Uptake (68 Ga-NODAGA-E[(cRGDyK)]₂) in Induced Myocardial Infarction and Stromal Cell Treatment in Minipigs

Diagnostics
Thomas RasmussenAndreas Kjær

Abstract

Angiogenesis is considered integral to the reparative process after ischemic injury. The αvβ₃ integrin is a critical modulator of angiogenesis and highly expressed in activated endothelial cells. 68Ga-NODAGA-E[(cRGDyK)]₂ (RGD) is a positron-emission-tomography (PET) ligand targeted towards αvβ₃ integrin. The aim was to present data for the uptake of RGD and correlate it with histology and to further illustrate the differences in angiogenesis due to porcine adipose-derived mesenchymal stromal cell (pASC) or saline treatment in minipigs after induction of myocardial infarction (MI). Three minipigs were treated with direct intra-myocardial injection of pASCs and two minipigs with saline. MI was confirmed by 82Rubidium (82Rb) dipyridamole stress PET. Mean Standardized Uptake Values (SUVmean) of RGD were higher in the infarct compared to non-infarct area one week and one month after MI in both pASC-treated (SUVmean: 1.23 vs. 0.88 and 1.02 vs. 0.86, p < 0.05 for both) and non-pASC-treated minipigs (SUVmean: 1.44 vs. 1.07 and 1.26 vs. 1.04, p < 0.05 for both). However, there was no difference in RGD uptake, ejection fractions, coronary flow reserves or capillary density in histology between the two groups. In summary, indications of a...Continue Reading

References

Apr 22, 1994·Science·P C BrooksD A Cheresh
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg
Sep 27, 2002·European Heart Journal·N A Y ChungG Y H Lip
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Jun 23, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Roland Haubner
Aug 13, 2008·The Journal of Surgical Research·Katja Schenke-LaylandW Robb Maclellan
Jun 10, 2011·British Medical Bulletin·Konstantinos Malliaras, Eduardo Marbán
Oct 1, 2011·Circulation Research·Patricia K NguyenJoseph C Wu
Oct 12, 2013·Journal of the American Heart Association·Rebecca D LevitW Robert Taylor
Jul 6, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Max KiugelAntti Saraste
Mar 5, 2016·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Thomas RasmussenPhilip Hasbak
Jun 3, 2017·European Heart Journal·Francisco Fernández-AvilésUNKNOWN Delivery, Navigation, Tracking and Assessment Subcommittee

❮ Previous
Next ❯

Citations

Sep 25, 2020·European Journal of Nuclear Medicine and Molecular Imaging·Marcus R MakowskiAmbros J Beer

❮ Previous
Next ❯

Methods Mentioned

BETA
Electrophoresis

Software Mentioned

SAS
Image J
Fiji
Axiovision
Syngo

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.