Angiogenesis within the duodenum of patients with cirrhosis is modulated by mechanosensitive Kruppel-like factor 2 and microRNA-126

Liver International : Official Journal of the International Association for the Study of the Liver
Karolina KobusMalgorzata Milkiewicz

Abstract

The mechanism involved in neovascularization in splanchnic circulation and the main trigger that induces angiogenesis in patients with cirrhosis are not fully recognized. To explore the involvement of flow sensitive lung Kruppel-like factor (KLF2), microRNA-126 (miR-126), angiopoietin-2 (Ang-2) and heme oxygenase-1 (HO-1) in modulation of vascular endothelial growth factor (VEGF) signalling that have a critical effect on growth of new blood vessels. Duodenal biopsies from 22 patients with cirrhosis and 10 controls were obtained during routine endoscopy. The process of angiogenesis was evaluated by a measurement of CD31 concentration, immunodetection of CD34 protein and estimation of capillary densities. Messenger RNA (mRNA) and protein expressions were analysed by real-time PCR, Western blot or ELISA respectively. Markers of angiogenesis (both, CD31 and CD34) were significantly enhanced in cirrhotic patients. In comparison to healthy controls, levels of Ang-2 and KLF-2 mRNAs as well as Ang-2, KLF-2, HO-1, VEGF protein expressions were considerably increased. Levels of sCD163, a surrogate marker of portal hypertension, correlated with levels of Ang-2, (P = 0.021) and VEGF (P = 0.009). The expression of miR-126, a KLF2-mediated r...Continue Reading

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Citations

Jul 3, 2015·Liver International : Official Journal of the International Association for the Study of the Liver·Eladio Rodriguez-DiazSatish K Singh
Jul 30, 2015·BioMed Research International·Ana Mondadori dos SantosValérie Metzinger-Le Meuth
May 18, 2016·Journal of Molecular and Cellular Cardiology·Dimitry A ChistiakovYuri V Bobryshev
Jan 27, 2018·Digestive Diseases and Sciences·Taiki AoyamaShinji Nagata
Jan 12, 2020·BioFactors·Xiaoyan ChenHeng Zhang
Jan 14, 2017·World Journal of Hepatology·Dmitry Victorovich GarbuzenkoDmitry Vladimirovich Belov
Dec 6, 2018·Revista Española De Enfermedades Digestivas : Organo Oficial De La Sociedad Española De Patología Digestiva·Na LiShiyao Chen

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