Angiogenic and astroglial responses to vascular endothelial growth factor administration in adult rat brain

Neuroscience
J M KrumJ M Rosenstein

Abstract

The effects of exogenous vascular endothelial growth factor (VEGF) on angiogenesis, blood-brain barrier permeability and astroglial proliferation in the adult rat CNS in situ were investigated. Recombinant human VEGF(165) (25 or 50 ng/ml) was delivered for up to 1 week using either intracerebral osmotic minipumps or less traumatic subdural gelatin sponge placement. By 3 days, VEGF delivery caused significantly increased cerebral angiogenesis (25 ng/ml was most effective) in both experimental models when compared to saline controls; VEGF infusion resulted in a 100% increase in an index of vascular proliferation, and gelatin sponge delivery produced a 65% increase. The blood-brain barrier hallmark endothelial glucose transporter-1 was not present in nascent vascular sprouts. Infusion of VEGF produced extensive protein leakage that persisted after saline-induced permeability was mostly resolved, while gelatin sponge administration caused milder barrier dysfunction. Administration of the angiogenic factor had unexpected proliferative effects on astroglia in both models, resulting in an 80-85% increase in mitotically active astroglia when compared to controls. Immunohistochemical results and semi-quantitative reverse transcriptase-p...Continue Reading

References

Sep 1, 1992·Neurochemical Research·W T NortonC F Brosnan
Sep 30, 1992·Biochemical and Biophysical Research Communications·B I TermanP Böhlen
Jan 1, 1991·Glia·M E HattenC A Mason
Nov 1, 1990·Journal of Neuropathology and Experimental Neurology·C EstradaP A Cancilla
Apr 15, 1987·The Journal of Comparative Neurology·J M Krum, J M Rosenstein
May 15, 1988·The Journal of Comparative Neurology·J M Krum, J M Rosenstein
Aug 1, 1988·Journal of Neurosurgery·G R CriscuoloE H Oldfield
Dec 1, 1985·Brain Research·P L RobertsonG W Goldstein
Sep 1, 1994·Stroke; a Journal of Cerebral Circulation·J KrupinskiJ M Wang
Jul 1, 1994·Brain Pathology·K H PlateW Risau
May 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D J DumontM L Breitman
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K G PetersL T Williams
Aug 1, 1994·Journal of Applied Physiology·S I HarikJ C LaManna
Apr 1, 1993·The American Journal of Physiology·W T MonacciE H Oldfield
Jan 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A L McCallN Lessov
Jan 15, 1996·The Journal of Clinical Investigation·E BrogiJ M Isner
Feb 1, 1996·Experimental Neurology·S B WirthK Unsicker
Aug 1, 1996·Experimental Neurology·J F Reilly, V G Kumari

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Citations

Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Ina M Wittko-SchneiderKarl H Plate
Mar 22, 2006·Journal of Neural Transmission·J V LafuenteB Mitre
Jun 19, 2013·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xian-Jun Ke, Jun-Jian Zhang
Feb 6, 2004·Brain Research. Developmental Brain Research·Alfia A KhaibullinaJanette M Krum
Jun 27, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Stuart M EllisonNicholas D Mazarakis
Aug 12, 2010·Journal of Neurotrauma·Olivera NesicPonnada A Narayana
Aug 16, 2006·Neuro-degenerative Diseases·Mariana PeharLuis Barbeito
May 3, 2006·Cerebrovascular Diseases·Olivia HurtadoMaría Angeles Moro
Sep 20, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Qi HaoGuo-Yuan Yang
Jun 19, 2003·The Journal of Clinical Investigation·Yunjuan SunDavid A Greenberg
Dec 18, 2008·Reproductive Biology and Endocrinology : RB&E·Chishimba N MowaGuichuan Hou
Nov 6, 2004·Reviews in the Neurosciences·Takao YasuharaIsao Date
Sep 30, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel P McCloskeyHelen E Scharfman
Nov 25, 2005·Journal of Neurosurgery·Marsha J Merrill, Edward H Oldfield
Jun 19, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jia-Jia Bi, Li Yi
Sep 14, 2013·Trends in Cognitive Sciences·Michelle W VossHenriette van Praag
Jan 16, 2016·Cellular and Molecular Life Sciences : CMLS·John BiancoAnne des Rieux
May 9, 2014·Journal of Neuropathology and Experimental Neurology·Daniela VirgintinoBarbara S Stonestreet
Oct 20, 2009·Experimental Neurology·Justin D LathiaThiruma V Arumugam
Apr 7, 2009·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Alper CelikSuna Omeroglu
Dec 10, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nina ManiJeffrey M Rosenstein
Jun 8, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Panya S ManoonkitiwongsaPatrick D Lyden
Sep 26, 2008·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Thomas HolzbachRiccardo E Giunta
Apr 15, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Dilaver KayaTurgay Dalkara
Sep 8, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Erik StorkebaumPeter Carmeliet

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