Angiogenic effects of adrenomedullin in ischemia and tumor growth

Circulation Research
Satoshi IimuroRyozo Nagai

Abstract

Adrenomedullin (AM) is a novel vasodilating peptide involved in the regulation of circulatory homeostasis and implicated in the pathophysiology of cardiovascular disease. We tested the hypothesis that AM also possesses angiogenic properties. Using laser Doppler perfusion imaging, we found that AM stimulated recovery of blood flow to the affected limb in the mouse hind-limb ischemia model. AM exerted this effect in part by promoting expression of vascular endothelial growth factor (VEGF) in the ischemic limb, and immunostaining for CD31 showed the enhanced flow to reflect increased collateral capillary density. By enhancing tumor angiogenesis, AM also promoted the growth of subcutaneously transplanted sarcoma 180 tumor cells. However, heterozygotic AM knockout mice (AM+/-) showed significantly less blood flow recovery with less collateral capillary development and VEGF expression than their wild-type littermates. Similarly, mice treated with AM22-52, a competitive inhibitor of AM, showed reduced capillary development, and growth of sarcoma 180 tumors was inhibited in AM+/- and AM22-52-treated mice. Notably, administration of VEGF or AM rescued blood flow recovery and capillary formation in AM+/- and AM22-52-treated mice. In cocu...Continue Reading

References

Nov 30, 1994·Biochemical and Biophysical Research Communications·H KanazawaT Takeda
Jul 29, 1994·Biochemical and Biophysical Research Communications·H WashimineT Eto
Mar 21, 1994·FEBS Letters·K KitamuraT Eto
Apr 30, 1993·Biochemical and Biophysical Research Communications·K KitamuraT Eto
May 1, 1996·American Heart Journal·K KobayashiT Eto
Feb 1, 1996·Journal of Hypertension·H KanoJ Yoshikawa
Oct 1, 1996·American Journal of Obstetrics and Gynecology·C J MacriF Cuttitta
Feb 1, 1997·Lancet·R Di IorioE V Cosmi
May 20, 1999·European Journal of Pharmacology·N ParameswaranP Nambi
Aug 11, 2000·In Vitro Cellular & Developmental Biology. Animal·E BonannoR F Nicosia
Sep 28, 2000·Molecular Human Reproduction·K HataK Miyazaki
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M SataR Nagai
Jun 8, 2002·Hypertension·Toshio NishikimiHiroaki Matsuoka
Aug 22, 2002·Journal of the National Cancer Institute·Alfredo MartínezFrank Cuttitta
Dec 13, 2002·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Monika A ZimanyiM Jane Black
Apr 8, 2003·Current Pharmaceutical Design·Peter SchratzbergerDouglas W Losordo
Jun 6, 2003·Biochemical and Biophysical Research Communications·Minami AbeRyozo Nagai

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Citations

Apr 4, 2006·Amino Acids·B Yanagawa, N Nagaya
Jun 2, 2005·Cancer Letters·Alfredo Martínez
Aug 19, 2008·Cardiovascular Research·Donghao JinNoritoshi Nagaya
Jul 25, 2009·International Immunology·Maya DamianovichYehuda Shoenfeld
May 27, 2005·Biology of Reproduction·Xiaoquan ZhangYuan Lin Dong
Sep 10, 2009·American Journal of Respiratory Cell and Molecular Biology·Arul VadivelBernard Thébaud
Dec 22, 2007·The Journal of Clinical Investigation·Yuka Ichikawa-ShindoTakayuki Shindo
Dec 22, 2007·The Journal of Clinical Investigation·Kimberly L Fritz-SixKathleen M Caron
Apr 16, 2005·Neuro-oncology·Balveen KaurErwin G Van Meir
Mar 23, 2013·PloS One·Susumu SakimotoNobuyuki Takakura
Oct 18, 2013·Cellular and Molecular Life Sciences : CMLS·Nobukazu MiyamotoKen Arai
May 11, 2013·Journal of Biosciences·F MolinaM L Del Moral
Sep 30, 2014·Journal of Molecular and Cellular Cardiology·Akihiro YamauchiTakayuki Shindo
Dec 6, 2014·Journal of Translational Medicine·Ignacio M LarráyozAlfredo Martínez
Jan 23, 2016·Progress in Retinal and Eye Research·Yasuhiro IesatoYasuo Yanagi
Jun 5, 2015·Journal of Atherosclerosis and Thrombosis·Teruhide KoyamaTakayuki Shindo
Apr 5, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Philippe MetellusL'Houcine Ouafik
May 13, 2008·International Journal of Cardiology·Hiroyuki AtsutaMasahiko Kurabayashi
Feb 13, 2014·International Journal of Cancer. Journal International Du Cancer·Liangjing WangDaniel C Chung
Jan 27, 2005·Pharmacology & Therapeutics·Shabaz A Hamid, Gary F Baxter
Jul 7, 2015·European Journal of Pharmacology·Johji Kato, Kazuo Kitamura
Nov 22, 2005·Regulatory Peptides·Ibane AbasoloAlfonso Calvo
Aug 23, 2005·Peptides·Domenico RibattiGastone G Nussdorfer
Apr 25, 2014·Circulation Journal : Official Journal of the Japanese Circulation Society·Tomohiko SatoEtsu Suzuki
Apr 11, 2015·The American Journal of Pathology·Yuichi ToriyamaTakayuki Shindo
Oct 22, 2013·Progress in Biophysics and Molecular Biology·Rajesh N Gacche, Rohan J Meshram
Apr 9, 2013·The American Journal of Pathology·Yasuhiro IesatoTakayuki Shindo
Jul 31, 2012·The American Journal of Pathology·Kentaro YudaYasuo Yanagi
Jun 17, 2014·Mathematical Biosciences·Amiya Ranjan Bhowmick, Sabyasachi Bhattacharya
Oct 28, 2005·British Journal of Cancer·L L NikitenkoR Bicknell

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