Angiogenic Potential of Cryopreserved Amniotic Membrane Is Enhanced Through Retention of All Tissue Components in Their Native State

Advances in Wound Care
Yi Duan-ArnoldAlla Danilkovitch

Abstract

Objective: Chronic wounds have inadequate microvasculature (or blood vessels), resulting in poor healing. Both fresh human amniotic membrane (hAM) containing viable cells and devitalized hAM have been shown to stimulate angiogenesis in chronic wounds. However, the importance of retaining viable endogenous cells on the angiogenic activity of hAM remains unknown. To understand their role, we compared the angiogenic potential of intact cryopreserved hAM containing viable cells (int-hAM) with devitalized cryopreserved hAM (dev-hAM). Approach: The effects of conditioned medium (CM) derived from int-hAM and dev-hAM on endothelial cell migration and tube formation were compared. Int-hAM and dev-hAM CM and tissues were tested for key angiogenic factors, such as vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and platelet-derived growth factor-BB (PDGF-BB) after 7 days in culture. The role of VEGF in int-hAM-mediated tube formation was analyzed through inhibition of its activity by anti-VEGF antibody. Results: CM from int-hAM showed greater endothelial cell recruitment and tube formation compared with dev-hAM. Significantly higher levels of VEGF were detected in int-hAM CM after 1 week compared with dev...Continue Reading

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Citations

Dec 21, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Eric L JohnsonGeorgina M Michael
Feb 22, 2018·Plastic and Reconstructive Surgery. Global Open·Rishi J GuptaNat R Gwilliam
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Dec 7, 2018·Expert Opinion on Biological Therapy·Jan AdamowiczTomasz Drewa
Sep 13, 2017·Diabetes Therapy : Research, Treatment and Education of Diabetes and Related Disorders·Irakoze LaurentQi Fu Li
Aug 20, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·John P McQuillingKatie C Mowry
Nov 19, 2021·International Wound Journal·Alzbeta SvobodovaKaterina Jirsova

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Methods Mentioned

BETA
biopsies
dissection
enzyme-linked immunosorbent assay
ELISAs
ELISA

Software Mentioned

ImageJ

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