Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration

Biomaterials Research
Beom-Su KimJun Lee

Abstract

Angiogenin (ANG) is a potent stimulator of angiogenesis. The aim of this study was to fabricate an ANG-loaded scaffold and to evaluate its angiogenic and osteogenic effects. In this study, we fabricated an ANG-loaded scaffold using bovine bone powder and fibrin glue. We then evaluated the structural, morphological, and mechanical properties of the scaffold and the in vitro release profile of ANG. Cell proliferation, viability, and adhesion were evaluated using endothelial cells in vitro, and angiogenesis and new bone formation were evaluated using a rabbit calvarial defect model in vivo. Micro-computed tomography imaging showed that the bone powder was uniformly distributed in the scaffold, and scanning electron microscopy showed that the bone powder was bridged by polymerized fibrin. The porosity and compressive strength of the scaffolds were ~60 % and ~0.9 MPa, respectively, and were not significantly altered by ANG loading. In vitro, at 7 days, approximately 0.4 μg and 1.3 μg of the ANG were released from the FB/ANG 0.5 and FB/ANG 2.0, respectively and sustained slow release was observed until 25 days. The released ANG stimulated cell proliferation and adherence and was not cytotoxic. Furthermore, in vivo implantation result...Continue Reading

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Citations

Nov 1, 2016·Journal of Biomedical Materials Research. Part a·Weiling YuKathryn E Uhrich
Jan 30, 2020·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Carlos Enrique Cuevas-SuárezEvandro Piva
Aug 28, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Junyu ChenAnjali P Kusumbe
Sep 6, 2020·International Journal of Molecular Sciences·André BuschMarcus Jäger
Dec 15, 2018·Journal of Periodontology·Klara JanjićHermann Agis

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
fluorescence imaging
scanning electron microscopy

Software Mentioned

CT
GraphPad
analyzer
GraphPad Prism
Skyscan

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