Angiopoietin-1-expressing adipose stem cells genetically modified with baculovirus nanocomplex: investigation in rat heart with acute infarction.

International Journal of Nanomedicine
Arghya PaulSatya Prakash

Abstract

The objective of this study was to develop angiopoietin-1 (Ang1)-expressing genetically modified human adipose tissue derived stem cells (hASCs) for myocardial therapy. For this, an efficient gene delivery system using recombinant baculovirus complexed with cell penetrating transactivating transcriptional activator TAT peptide/deoxyribonucleic acid nanoparticles (Bac-NP), through ionic interactions, was used. It was hypothesized that the hybrid Bac- NP(Ang1) system can efficiently transduce hASCs and induces favorable therapeutic effects when transplanted in vivo. To evaluate this hypothesis, a rat model with acute myocardial infarction and intramyocardially transplanted Ang1-expressing hASCs (hASC-Ang1), genetically modified by Bac-NP(Ang1), was used. Ang1 is a crucial pro-angiogenic factor for vascular maturation and neovasculogenesis. The released hAng1 from hASC-Ang1 demonstrated profound mitotic and anti-apoptotic activities on endothelial cells and cardiomyocytes. The transplanted hASC-Ang1 group showed higher cell retention compared to hASC and control groups. A significant increase in capillary density and reduction in infarct sizes were noted in the infarcted hearts with hASC-Ang1 treatment compared to infarcted hearts...Continue Reading

References

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Citations

Sep 11, 2014·Journal of Cardiovascular Translational Research·Lina ChenJianguo Xu
Sep 24, 2015·Advanced Drug Delivery Reviews·Jin HanByung-Soo Kim
Jun 2, 2015·Revista Española De Cardiología·Lina BadimonGemma Vilahur
Nov 8, 2018·Stem Cells International·Kar Wey YongArindom Sen
Sep 15, 2020·Frontiers in Bioengineering and Biotechnology·Yuting SunZhenjie Liu
Mar 12, 2018·ACS Biomaterials Science & Engineering·Ranjith Kumar KankalaAi-Zheng Chen
Oct 8, 2021·Applied Microbiology and Biotechnology·Alexandra Marisa TargovnikMariano Nicolás Belaich

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Methods Mentioned

BETA
transfection
enzyme-linked immunosorbent assay
ELISA
Assay
PCR
electrophoresis

Software Mentioned

Zeta Potential Analyzer
GraphPad
GraphPad Prism
ZetaPlus Particle Sizer
ImageJ

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