Abstract
Retinae of aged humans show signs of vascular regression. Vascular regression involves a mismatch between Angiopoietin-2 (Ang-2) and vascular endothelial growth factor (VEGF) expression. We used heterozygous Ang-2 deficient (Ang2LacZ) mice to evaluate murine retinal vascular changes and gene expression of growth factors. Vascular changes were assessed by quantitative retinal morphometry and gene expression levels of growth factors were measured by quantitative PCR. The numbers of endothelial cells and pericytes did not change in the Ang2LacZ retinae with age, whereas they decreased throughout the age spectrum studied in the wild type retinae. Moreover, vascular regression significantly decelerated in the heterozygous Ang2LacZ retinae (200% to 1 month), while the formation of acellular capillaries was significantly increased at 13 months in the wild type retinae (340% to 1 month). Gene expression analysis revealed that VEGF, Ang-1, PDGF-B and Ang2 mRNA levels were decreased in the wild type retinae at 9 month of age. However, the decrease of Ang-2 was smaller compared with other genes. While VEGF levels dropped in wild type mice up to 60% compared to 1 month, VEGF increased in heterozygous Ang-2 deficient retinae at an age of 9 ...Continue Reading
References
Jul 1, 1979·Microvascular Research·H J Glatt, P Henkind
May 1, 1996·Nature Medicine·H P HammesK T Preissner
Jul 4, 1997·Science·P C MaisonpierreG D Yancopoulos
Jul 11, 1997·Science·P LindahlC Betsholtz
Jul 4, 1997·Science·D Hanahan
Jan 5, 2000·Science·G ThurstonD M McDonald
Mar 31, 2000·Nature Medicine·G ThurstonG D Yancopoulos
Jul 27, 2000·Journal of Cellular Physiology·S F HackettP A Campochiaro
Mar 3, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D H KangR J Johnson
May 8, 2001·Cells, Tissues, Organs·G Allt, J G Lawrenson
May 10, 2002·The American Journal of Pathology·Antonia M JoussenAnthony P Adamis
Aug 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ivan B LobovRichard A Lang
Aug 10, 2002·The EMBO Journal·Maria EngeChrister Betsholtz
Sep 28, 2002·Diabetes·Hans-Peter HammesUrban Deutsch
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Sep 1, 1961·Archives of Ophthalmology·D G COGANT KUWABARA
May 1, 1961·Archives of Ophthalmology·T KUWABARAD G COGAN
Mar 6, 2004·Circulation Research·Fabienne BaffertDonald M McDonald
Mar 30, 2004·Diabetes·Hans-Peter HammesUrban Deutsch
May 5, 2005·The Journal of Pharmacology and Experimental Therapeutics·Fiorentina RoviezzoAndreas Papapetropoulos
Sep 17, 2005·Circulation Research·Annika ArmulikChrister Betsholtz
Oct 11, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J HoffmannH-P Hammes
Nov 18, 2005·Experimental Gerontology·A Wagatsuma
Jan 3, 2006·Neurobiology of Aging·Suzanne HughesTailoi Chan-Ling
Jan 4, 2007·Thrombosis and Haemostasis·Yuxi FengHans-Peter Hammes
Citations
Apr 10, 2013·Pflügers Archiv : European journal of physiology·Frederick PfisterHans-Peter Hammes
Jul 10, 2010·Bioinformatics·Jianfei HuJiang Qian
Feb 26, 2011·Fluids and Barriers of the CNS·Drew BonkowskiPaula Dore-Duffy
May 2, 2014·PloS One·Yanji ZhuBing Xie
Aug 6, 2013·Cellular and Molecular Life Sciences : CMLS·Kristina Y Aguilera, Rolf A Brekken
Dec 17, 2015·Omics : a Journal of Integrative Biology·Akanksha Gupta, Sonika Bhatnagar
Jun 22, 2010·Experimental Physiology·C F BentoP Pereira
Mar 16, 2011·Brain Research·Girriso F Benderro, Joseph C Lamanna
Mar 23, 2017·Journal of Cardiovascular Pharmacology·Nicholas A Flavahan
May 23, 2017·Nature Reviews. Drug Discovery·Pipsa SaharinenKari Alitalo
Jan 24, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Konstantin GaengelChrister Betsholtz
Apr 23, 2015·Acta Diabetologica·Yi QiuYuxi Feng
Oct 2, 2019·Investigative Ophthalmology & Visual Science·Evan A ThackaberryVladimir Bantseev
Dec 23, 2017·PloS One·Nasim JamaliNader Sheibani