Angiopoietin 2 promotes angiogenesis in tissue-engineered bone and improves repair of bone defects by inducing autophagy

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Jian YinXinhui Liu

Abstract

Angiogenesis plays a key role in the repair of large segmental bone defects with tissue-engineered bones. However, there is no effective method of promoting angiogenesis in tissue-engineered bone. Both angiopoietin 2 (Ang2) and autophagy have been shown to be involved in angiogenesis, but their roles in angiogenesis of tissue-engineered bone remains unknown. In this in vivo study, a radius bone defect was created in New Zealand white rabbits, which were then treated by implantation of a hydroxyapatite/collagen scaffold followed by injection of different concentrations of Ang2. Expression of the autophagic modulators microtubule-associated protein 1 light chain 3 (LC3), Beclin-1, and SQSTM1/P62 were measured via western blotting, while the angiogenic modulators VEGF and CD31 were detected by western blotting and immunohistochemistry, respectively. X-ray imaging combined with general observation was used to evaluate bone defect healing. Expression of LC3 -I/LC3-II, Beclin-1, VEGF, and CD31 in the callus area increased and SQSTM1/p62 decreased in a dose-dependent manner with increasing Ang2 concentration. In the group treated with a high concentration of Ang2, the new callus grew well, accompanied by remarkable angiogenesis, leadi...Continue Reading

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Citations

Jul 9, 2020·Tissue Engineering. Part B, Reviews·Maximilian M MengerTina Histing
May 22, 2019·Cells·Racheal G AkwiiConstantinos M Mikelis
Dec 19, 2019·Tissue Engineering. Part C, Methods·Patricia CarlisleTeja Guda
Apr 23, 2020·International Journal of Molecular Sciences·Viviana di GiacomoSilvia Sancilio
Apr 12, 2019·Archives of Oral Biology·Alejandro I Lorenzo-PousoYago Leira

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