Angiopoietin-like 8 (Angptl8) controls adipocyte lipolysis and phospholipid composition

Chemistry and Physics of Lipids
Raghavendra MysoreP A Nidhina Haridas

Abstract

Angiopoietin-like 8 (Angptl8) inhibits lipolysis in the circulation together with Angplt3 and controls post-prandial fat storage in white adipose tissue (WAT). It is strongly induced by insulin in vivo in WAT and in vitro in adipocytes. In this study we addressed the function of Angptl8 in adipocytes by its stable lentivirus-mediated knock-down in 3T3-L1 cells, followed by analyses of triglyceride (TG) storage, lipid droplet (LD) morphology, the cellular lipidome, lipolysis, and gene expression. Depletion of Angptl8 did not drastically affect the adipocyte differentiation of 3T3-L1 cells but resulted in a moderate (18-19%) reduction of stored TGs. The lipidome analysis revealed a reduction of alkyl- phosphatidylcholines (PCs) and phosphatidylethanolamine (PE) plasmalogens, as well as saturated PCs and PEs. Importantly, the Angptl8 depleted cells displayed enhanced lipolysis as measured by release of non-esterified fatty acids (NEFAs). Consistently, mRNAs encoding Angptl4 and Leptin, which facilitate lipolysis, as well as Cpt1a, Cpt1b, and Pgc-1α involved in FA oxidation, were elevated. The Angptl8 mRNA itself was suppressed by pharmacologic treatments inducing lipolysis: stimulation with the β-adrenergic agonist isoproterenol o...Continue Reading

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Citations

Sep 25, 2017·The Journal of Clinical Endocrinology and Metabolism·Raghavendra MysoreP A Nidhina Haridas
Oct 5, 2020·Progress in Lipid Research·Mohamed Abu-FarhaMarc Prentki
May 24, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xin SuBin Wang
Sep 29, 2021·Open Biology·Chang GuoGuoyue Yuan

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