Angiopoietin-like protein 2 promotes inflammatory conditions in the ligamentum flavum in the pathogenesis of lumbar spinal canal stenosis by activating interleukin-6 expression

European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
Takayuki NakamuraHiroshi Mizuta

Abstract

Chronic inflammation is thought to cause ligamentum flavum (LF) degeneration and hypertrophy in lumbar spinal canal stenosis (LSCS). Angiopoietin-like protein 2 (Angptl2) is highly expressed in hypertrophied LF. Because Angptl2 regulates interleukin-6 (IL-6) expression in various tissues, we investigated whether IL-6 is expressed in hypertrophied LF and, if so, does Angptl2 induce IL-6 expression in LF fibroblasts. LF tissue was obtained from LSCS patients and non-LSCS patients. Polymerase chain reaction (PCR) for Angptl2 and IL-6 genes and immunohistochemistry for IL-6 protein were performed in LF tissue. Fibroblasts from LF tissue were used for in vitro experiments. Expression of integrin α5β1 (an Angptl2 receptor) and Angptl2 binding to receptors on LF fibroblasts were examined by fluorescence-activated cell sorter analysis and cell adhesion assays. After Angptl2 recombinant protein treatment, NF-κB activation and IL-6 expression in LF fibroblasts were investigated by immunocytochemistry, PCR, and enzyme-linked immunosorbent assay. IL-6 mRNA expression was increased in hypertrophied LF tissue from LSCS patients and positively correlated with LF thickness and Angptl2 mRNA expression. IL-6 protein was highly expressed in LF fi...Continue Reading

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Citations

Nov 26, 2015·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Michel Benoist
Oct 6, 2015·Biochemical and Biophysical Research Communications·Masato UmikawaCheng Cheng Zhang
Sep 17, 2016·Scoliosis and Spinal Disorders·Prudence Wing Hang CheungJason Pui Yin Cheung
Apr 6, 2017·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Jung Sub LeeTae Sik Goh
Dec 19, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kyuha ChongHong Joo Moon
Jul 25, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sunghyeok YeJunseok W Hur
Apr 9, 2018·Pharmacological Reports : PR·Hong-Bo XiaoZhi-Liang Sun
Jul 2, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chao SunXinhui Liu
Feb 10, 2017·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Junseok W HurJunho K Hur
Sep 5, 2020·Biological Procedures Online·Cheng-Li LinTing-Yuan Tu
Nov 27, 2017·Spine Surgery and Related Research·Taiki MoriKen Watanabe
Oct 5, 2019·Cartilage·Mami TakanoKotaro Tanimoto
Oct 29, 2021·Journal of Periodontology·Chenxi JiangZhengguo Cao

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