Angiopoietin-like protein 3 regulates the motility and permeability of podocytes by altering nephrin expression in vitro

Biochemical and Biophysical Research Communications
Xia GaoXiliang Zha

Abstract

It is well known that podocyte injury plays a vital role in massive proteinuria. The increase of podocyte motility results in podocyte foot process (FP) effacement, a typical form of podocyte injury. Our previous studies demonstrated that glomerular podocytes can express angiopoietin-like protein 3 (ANGPTL3) and that the increase of ANGPTL3 in dysfunctional glomerulus is correlated with podocyte FP effacement. Little is known, however, about the role of ANGPTL3 in podocyte injury. In this study, we investigated ANGPTL3's effect on the motility and permeability of podocytes and on the expression of nephrin, a key molecule in podocytes. By scrape-wound and transwell migration assay, we found that ANGPTL3 over-expression significantly increased podocyte motility, whereas after ANGPTL3 knockdown by RNA interference, motility remained the same as that of the control group. Adriamycin (ADR) treatment significantly promoted podocyte motility. However, the same dose of ADR treatment could not promote motility after the knockdown of ANGPTL3. In addition, we assayed the diffusion of FITC-BSA across the podocytes' monolayer to investigate whether ANGPTL3 could promote protein loss by means of an increase in podocyte motility. The results ...Continue Reading

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Citations

Jan 8, 2014·Nature Reviews. Nephrology·Jochen ReiserChristian Faul
Apr 2, 2014·Biochemical and Biophysical Research Communications·So-Hyun LeeMin Jung Kim
Feb 25, 2015·Pediatric Research·Junchao LiuHong Xu
May 5, 2012·Metabolism: Clinical and Experimental·Alessandra Dei Cas, Luigi Gnudi
May 20, 2019·Lipids in Health and Disease·Xia GaoQingju Liu
Aug 22, 2018·Investigative Ophthalmology & Visual Science·Na WangZhi Zheng
Mar 19, 2014·The International Journal of Artificial Organs·Dana MahmoodBernd Stegmayr
Dec 15, 2020·American Journal of Physiology. Renal Physiology·Wenjun GaoFan Fan

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