Abstract
To investigate the effects of angiotensin-converting enzyme 2 (ACE2) activation on pulmonary arterial cell apoptosis during pulmonary vascular remodeling associated with pulmonary arterial hypertension (PAH) and to elucidate potential mechanisms related to Hippo signaling. PAH model was developed by injecting monocrotaline combined with left pneumonectomy using Sprague-Dawley rat. Then, resorcinolnaphthalein (Res; ACE2 activator), MLN-4760 (ACE2 inhibitor), A-779 (Mas inhibitor), and 4-((5,10-dimethyl-6-oxo-6,10-dihydro-5H-pyrimido[5,4-b]thieno[3,2-e][1,4]diazepin-2-yl)amino) benzenesulfonamide (XMU-MP-1; MST1/2 inhibitor) were administered via continuous subcutaneous or intraperitoneal injection for 3 weeks. Animals were randomly divided into six groups: control, PAH, PAH+Res, PAH+Res+MLN-4760, PAH+Res+A-779, and PAH+Res+XMU-MP-1. On 21 day, hemodynamics and pathologic lesions were evaluated. Apoptosis and apoptosis-associated proteins were detected by TUNEL and western blotting. ACE2 activity and Hippo pathway components including large tumor suppressor 1 (LATS1), Yes-associated protein (Yap), and phosphorylated Yap (p-Yap) were investigated by fluorogenic peptide assays and western blotting. In the PAH models, the mean pulmo...Continue Reading
References
Jun 6, 1986·Cell·D YoungM Wigler
Sep 10, 2003·Circulation·Toshihiko NishimuraPeter N Kao
Jun 3, 2005·The Journal of Clinical Investigation·M Sean McMurtryEvangelos D Michelakis
Sep 16, 2005·Endothelium : Journal of Endothelial Cell Research·Enrique ArciniegasRichard Ramírez
Jul 28, 2006·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Emin Gurbanov, Xiao Shiliang
Jun 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sebastien BonnetEvangelos D Michelakis
Nov 3, 2007·Genes & Development·Bin ZhaoKun-Liang Guan
Dec 26, 2007·The Journal of Biological Chemistry·Yawei HaoXiaolong Yang
Feb 28, 2009·American Journal of Respiratory and Critical Care Medicine·Anderson J FerreiraMohan K Raizada
Apr 9, 2009·Cancer Research·Ming-Yang WangMin-Liang Kuo
Jul 1, 2009·Hypertension·Yoriko YamazatoMohan K Raizada
Oct 15, 2009·Respiratory Research·Seiichiro SakaoNorbert F Voelkel
Sep 14, 2010·American Journal of Respiratory and Critical Care Medicine·Serpil ErzurumTimothy M Moore
Dec 9, 2010·Development·Georg Halder, Randy L Johnson
Jan 11, 2011·Current Opinion in Pharmacology·Vinayak ShenoyMohan K Raizada
Jul 21, 2011·Journal of the American Society of Hypertension : JASH·Wilbert S AronowDeborah J Wesley
Aug 7, 2012·Cell·Fa-Xing YuKun-Liang Guan
Oct 27, 2012·The European Respiratory Journal·Bruce D UhalMaria Molina-Molina
Dec 19, 2012·Cell·Joseph RosenbluhWilliam C Hahn
May 9, 2013·Lung·Gang LiLizhong Sun
Nov 26, 2013·Circulation·Dmitry A GoncharovElena A Goncharova
Nov 14, 2014·Cell Death & Disease·D O WennmannH Pavenstädt
May 23, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Lifang WangTieying Sun
Jun 18, 2015·Cancer Science·Yi LuoHiroki Kuniyasu
Aug 1, 2015·Molecular Medicine·Yanping LiuPanpan Hao
Oct 4, 2015·Molecular Cancer Research : MCR·Ursula Ehmer, Julien Sage
Nov 14, 2015·Cell Reports·Thomas BerteroStephen Y Chan
Jan 6, 2016·Genes & Development·Zhipeng MengKun-Liang Guan
Mar 5, 2016·The Lancet. Respiratory Medicine·Annie Christine LajoieSteeve Provencher
Apr 28, 2016·American Journal of Respiratory and Critical Care Medicine·Tatiana V KudryashovaElena A Goncharova
Jul 28, 2016·Oncology Reports·Qijian ChengYun Feng
Aug 19, 2016·Science Translational Medicine·Fuqin FanDawang Zhou
Aug 23, 2016·The Journal of Clinical Investigation·Thomas BerteroStephen Y Chan
Apr 4, 2017·British Journal of Pharmacology·Jinlong HeDing Ai
Jun 10, 2017·Pulmonary Circulation·Olivier BoucheratSebastien Bonnet
Citations
Dec 31, 2019·Experimental and Therapeutic Medicine·Xinhao WangWei Guo
May 27, 2020·Biology of Sex Differences·Catherine GebhardSabra L Klein
Jan 5, 2021·Cardiovascular Drugs and Therapy·Feng ZhangYing Han
Jan 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Murat Oz, Dietrich Ernst Lorke
Oct 17, 2020·Clinical Science·Qiuhong LiMohan K Raizada
Apr 3, 2021·Molecular and Cellular Biochemistry·Qianqian ZhangManxiang Li
May 6, 2021·Respiratory Research·Yanxia WangZhichao Li
Jun 27, 2021·The FEBS Journal·Jiahong XieHongfeng Jiang
Jul 27, 2021·Frontiers in Physiology·Xinhao WangWei Guo
Aug 29, 2021·Journal of Molecular Histology·Juan-Juan SongJiu-Chang Zhong
Oct 22, 2020·Current Drug Targets·Cristina VieiraAna Cristina Simões E Silva