Angiotensin-converting enzyme 2 activation suppresses pulmonary vascular remodeling by inducing apoptosis through the Hippo signaling pathway in rats with pulmonary arterial hypertension

Clinical and Experimental Hypertension : CHE
Daole YanYinglong Liu

Abstract

To investigate the effects of angiotensin-converting enzyme 2 (ACE2) activation on pulmonary arterial cell apoptosis during pulmonary vascular remodeling associated with pulmonary arterial hypertension (PAH) and to elucidate potential mechanisms related to Hippo signaling. PAH model was developed by injecting monocrotaline combined with left pneumonectomy using Sprague-Dawley rat. Then, resorcinolnaphthalein (Res; ACE2 activator), MLN-4760 (ACE2 inhibitor), A-779 (Mas inhibitor), and 4-((5,10-dimethyl-6-oxo-6,10-dihydro-5H-pyrimido[5,4-b]thieno[3,2-e][1,4]diazepin-2-yl)amino) benzenesulfonamide (XMU-MP-1; MST1/2 inhibitor) were administered via continuous subcutaneous or intraperitoneal injection for 3 weeks. Animals were randomly divided into six groups: control, PAH, PAH+Res, PAH+Res+MLN-4760, PAH+Res+A-779, and PAH+Res+XMU-MP-1. On 21 day, hemodynamics and pathologic lesions were evaluated. Apoptosis and apoptosis-associated proteins were detected by TUNEL and western blotting. ACE2 activity and Hippo pathway components including large tumor suppressor 1 (LATS1), Yes-associated protein (Yap), and phosphorylated Yap (p-Yap) were investigated by fluorogenic peptide assays and western blotting. In the PAH models, the mean pulmo...Continue Reading

References

Jun 3, 2005·The Journal of Clinical Investigation·M Sean McMurtryEvangelos D Michelakis
Sep 16, 2005·Endothelium : Journal of Endothelial Cell Research·Enrique ArciniegasRichard Ramírez
Jul 28, 2006·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Emin Gurbanov, Xiao Shiliang
Jun 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sebastien BonnetEvangelos D Michelakis
Dec 26, 2007·The Journal of Biological Chemistry·Yawei HaoXiaolong Yang
Feb 28, 2009·American Journal of Respiratory and Critical Care Medicine·Anderson J FerreiraMohan K Raizada
Sep 14, 2010·American Journal of Respiratory and Critical Care Medicine·Serpil ErzurumTimothy M Moore
Dec 9, 2010·Development·Georg Halder, Randy L Johnson
Jan 11, 2011·Current Opinion in Pharmacology·Vinayak ShenoyMohan K Raizada
Oct 27, 2012·The European Respiratory Journal·Bruce D UhalMaria Molina-Molina
May 23, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Lifang WangTieying Sun
Jun 18, 2015·Cancer Science·Yi LuoHiroki Kuniyasu
Oct 4, 2015·Molecular Cancer Research : MCR·Ursula Ehmer, Julien Sage
Jan 6, 2016·Genes & Development·Zhipeng MengKun-Liang Guan
Mar 5, 2016·The Lancet. Respiratory Medicine·Annie Christine LajoieSteeve Provencher
Apr 28, 2016·American Journal of Respiratory and Critical Care Medicine·Tatiana V KudryashovaElena A Goncharova
Aug 19, 2016·Science Translational Medicine·Fuqin FanDawang Zhou
Aug 23, 2016·The Journal of Clinical Investigation·Thomas BerteroStephen Y Chan
Jun 10, 2017·Pulmonary Circulation·Olivier BoucheratSebastien Bonnet

❮ Previous
Next ❯

Citations

Dec 31, 2019·Experimental and Therapeutic Medicine·Xinhao WangWei Guo
May 27, 2020·Biology of Sex Differences·Catherine GebhardSabra L Klein
Jan 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Murat Oz, Dietrich Ernst Lorke
Oct 17, 2020·Clinical Science·Qiuhong LiMohan K Raizada
Jun 27, 2021·The FEBS Journal·Jiahong XieHongfeng Jiang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis