Angiotensin-converting enzyme-2 overexpression improves atrial remodeling and function in a canine model of atrial fibrillation

Journal of the American Heart Association
Tingquan ZhouYuehui Yin

Abstract

Atrial fibrosis is an important factor in initiating and maintaining atrial fibrillation. The purpose of this study was to test the hypothesis that atrial angiotensin-converting enzyme-2 (ACE2) overexpression might inhibit atrial collagen accumulation and improve atrial remodeling in a canine atrial pacing model. Thirty-two mongrel dogs of both genders were divided randomly into 4 groups: sham-operated, control, gene therapy with adenovirus-enhanced green fluorescent protein (Ad-EGFP), and gene therapy with Ad-ACE2. All of the dogs in the control, Ad-EGFP, and Ad-ACE2 groups were paced at 450 bpm for a period of 14 days. The dogs in the sham group were instrumented without pacing. After 2 weeks, all of the dogs underwent a thoracotomy operation and received epicardial gene painting. On post-gene transfer day 21, the animals underwent electrophysiology, histology, and molecular studies. The percentage of fibrosis in the Ad-ACE2 group was markedly lower than the percentage in the control and Ad-EGFP groups. Compared with the other groups, ACE2 expression was increased significantly in the Ad-ACE2 group. Compared with the sham and Ad-ACE2 groups, the expression levels of transforming growth factor-β1 and Smad3 were significantly h...Continue Reading

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Citations

Oct 16, 2016·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Tomos E WaltersLouise M Burrell
Dec 10, 2019·Journal of Cellular and Molecular Medicine·Juan WangYao Li
Sep 11, 2020·Circulation Journal : Official Journal of the Japanese Circulation Society·Yu-Feng HuShih-Ann Chen
Oct 6, 2020·Expert Opinion on Drug Discovery·Chun-Ka Wong, Hung-Fat Tse
Oct 17, 2020·Clinical Science·Qiuhong LiMohan K Raizada

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Methods Mentioned

BETA
transgenic
electrophoresis
PCR
ELISA

Software Mentioned

GraphPad
GraphPad Prism
SPSS

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