Abstract
Atrial fibrosis is an important factor in initiating and maintaining atrial fibrillation. The purpose of this study was to test the hypothesis that atrial angiotensin-converting enzyme-2 (ACE2) overexpression might inhibit atrial collagen accumulation and improve atrial remodeling in a canine atrial pacing model. Thirty-two mongrel dogs of both genders were divided randomly into 4 groups: sham-operated, control, gene therapy with adenovirus-enhanced green fluorescent protein (Ad-EGFP), and gene therapy with Ad-ACE2. All of the dogs in the control, Ad-EGFP, and Ad-ACE2 groups were paced at 450 bpm for a period of 14 days. The dogs in the sham group were instrumented without pacing. After 2 weeks, all of the dogs underwent a thoracotomy operation and received epicardial gene painting. On post-gene transfer day 21, the animals underwent electrophysiology, histology, and molecular studies. The percentage of fibrosis in the Ad-ACE2 group was markedly lower than the percentage in the control and Ad-EGFP groups. Compared with the other groups, ACE2 expression was increased significantly in the Ad-ACE2 group. Compared with the sham and Ad-ACE2 groups, the expression levels of transforming growth factor-β1 and Smad3 were significantly h...Continue Reading
References
Mar 1, 1994·Journal of Molecular and Cellular Cardiology·K T WeberJ P Cleutjens
Jul 1, 1997·Journal of Molecular and Cellular Cardiology·S E Campbell, L C Katwa
Sep 17, 1998·Journal of Molecular and Cellular Cardiology·Y SunF J Ramires
Jul 7, 1999·Circulation·D LiS Nattel
May 12, 2000·Japanese Heart Journal·Y SekoR Nagai
Sep 2, 2000·Circulation Research·M DonoghueS Acton
Dec 5, 2000·The Journal of Biological Chemistry·B RazaniM P Lisanti
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Jun 21, 2002·Nature·Michael A CrackowerJosef M Penninger
Dec 14, 2002·Circulation Research·Ichiro ManabeRyozo Nagai
Jun 25, 2003·Journal of the American College of Cardiology·Koichiro KumagaiKeijiro Saku
Feb 20, 2004·Japanese Heart Journal·Yoshiyuki HirayamaTeruo Takano
Oct 23, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Erik R OlsonJ Gary Meszaros
Nov 13, 2004·Circulation Research·Kui ChenJacob Joseph
Jan 12, 2005·Circulation·Kan KikuchiJ Kevin Donahue
Apr 9, 2005·Circulation Research·Andreas S BarthMichael Nabauer
Dec 31, 2005·Basic Research in Cardiology·A BoldtSt Dhein
May 31, 2006·The Journal of Thoracic and Cardiovascular Surgery·Giovanni MariscalcoAndrea Sala
Jan 20, 2007·Circulation Research·Sophie CardinStanley Nattel
May 19, 2007·Trends in Endocrinology and Metabolism : TEM·Rajesh KumarKenneth M Baker
Oct 3, 2007·Cardiovascular Drugs and Therapy·Stavros I ChrysostomakisPanos E Vardas
Feb 2, 2008·Circulation Research·Iain L O Buxton, Dayue Duan
Sep 16, 2009·Journal of Molecular and Cellular Cardiology·Jason PellmanFarah Sheikh
Mar 18, 2010·Clinical Cardiology·Hua XiaoXi Wang
Sep 8, 2011·Journal of Pharmacological Sciences·Bai LeiAkira Nishiyama
Nov 2, 2011·Circulation·Jared W MagnaniEmelia J Benjamin
Oct 30, 2012·Lancet·Geoffrey LeeJonathan M Kalman
Jul 19, 2013·European Heart Journal·Lukas Kappenberger
Dec 3, 2014·Journal of Molecular and Cellular Cardiology·Juan SongYuehua Li
Citations
Sep 17, 2016·Life Sciences·Lei LaiFeng Ling
Oct 16, 2016·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Tomos E WaltersLouise M Burrell
Dec 10, 2019·Journal of Cellular and Molecular Medicine·Juan WangYao Li
Nov 8, 2017·Oncotarget·Tingquan ZhouChangqian Wang
Sep 11, 2020·Circulation Journal : Official Journal of the Japanese Circulation Society·Yu-Feng HuShih-Ann Chen
Apr 3, 2019·Heart and Vessels·Xiang QuHao Zhou
Oct 6, 2020·Expert Opinion on Drug Discovery·Chun-Ka Wong, Hung-Fat Tse
Feb 20, 2021·BMC Cardiovascular Disorders·Cheng LiuYan Shen
Oct 17, 2020·Clinical Science·Qiuhong LiMohan K Raizada
Sep 16, 2020·BMJ Open·Hajira Dambha-MillerSimon J Griffin