PMID: 9525548Apr 3, 1998Paper

Angiotensin-converting enzyme genotypes and risk for myocardial infarction in women

Journal of the American College of Cardiology
J L AndersonR H Ward

Abstract

We tested for an association between the angiotensin-converting enzyme (ACE) DD polymorphic genotype and myocardial infarction (MI) in a sample group composed exclusively of women. The human ACE gene occurs with either an insertion (I allele) or a deletion (D allele) of a 287-base pair (bp) Alu element. Part of the variance in serum ACE levels may be accounted for by this polymorphism. Also, the DD genotype has been associated with an increased risk of MI in predominantly male populations. However, the risk in women is poorly defined. Genomic DNA was extracted from buffy coat blood using a phenol/chloroform method. Angiotensin-converting enzyme alleles were identified using primers to bracket the insertion region in intron 16. Amplification using polymerase chain reaction allowed identification of a 490-bp (I allele) or a 190-bp (D allele) product, or both. Allelic and genotypic frequencies in control subjects were similar to those reported in mostly male populations, and frequencies of genotypes were in the Hardy-Weinberg equilibrium. In contrast, the distribution of genotypes in patients with MI diverged from the equilibrium. Specifically, DD genotypic frequency was increased in women with (n = 141) versus without (n = 338) a...Continue Reading

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Citations

Nov 22, 2001·Maturitas·F MassartM L Brandi
Mar 26, 1999·Journal of the American College of Cardiology·J L AndersonJ F Carlquist
Apr 5, 2003·The International Journal of Biochemistry & Cell Biology·Jop H van Berlo, Yigal M Pinto
Dec 21, 2000·European Journal of Clinical Investigation·F KeeA E Evans
Nov 7, 2003·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·L CovoloG Nardi
Nov 9, 2005·International Journal of Cardiology·Julie MéthotPaul Poirier
Oct 31, 2007·American Heart Journal·Benjamin D HorneUNKNOWN Intermountain Heart Collaborative Study Group
Dec 15, 2010·Journal of Clinical Pharmacology·Bettina A HamelinJacques Turgeon
Jun 3, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·P StrattaG Piccoli

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