Angiotensin-converting enzyme in Spodoptera littoralis: molecular characterization, expression and activity profile during development

Insect Biochemistry and Molecular Biology
Els LemeireGuy Smagghe

Abstract

The characterization of the full-length angiotensin-converting enzyme (ACE) cDNA sequence of the lepidopteran Spodoptera littoralis is reported in this study. The predicted open reading frame encodes a 647 amino acids long protein (SlACE) and shows 63.6% identity with the Bombyx mori ACE sequence. A 3D-model, consisting of 26 alpha-helices and three beta-sheets, was predicted for the sequence. SlACE expression was studied in the embryonic, larval and pupal stages of S. littoralis and in different tissues of the last larval stage by reverse-transcribed PCR. This revealed that the gene is expressed throughout the life cycle and especially in brain, gut and fat body tissue of the last stage. These results are in agreement with a role of ACE in the metabolism of neuropeptides and gut hormones. In addition, ACE activity has been studied in more detail during development, making use of a fluorescent assay. High ACE peptidase activity coincides with every transition state, from embryo to larva, from larva to larva and from larva to pupa. A peak value in activity occurs during the early pupal stage. These results indicate the importance of SlACE during metamorphosis and reveal the high correlation of ACE activity with the insect's deve...Continue Reading

References

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Citations

Oct 19, 2010·FEBS Letters·Kálmán SomogyiIstván Andó
Mar 7, 2015·Scientific Reports·Wei WangFeng Cui
Oct 25, 2008·Archives of Insect Biochemistry and Physiology·Els LemeireGuy Smagghe
Jun 21, 2016·General and Comparative Endocrinology·Tewodros Firdissa DuressaRoger Huybrechts
Nov 23, 2017·Scientific Reports·Yong ZhangJulian Chen

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