Angiotensin II and angiotensin-(1-7) effects on free cytosolic sodium, intracellular pH, and the Na(+)-H+ antiporter in vascular smooth muscle

Hypertension
M YeD Batlle

Abstract

The aim of the present study was to define the effects of angiotensin II (Ang II) and Ang-(1-7) on free cytosolic Na+ (Na+i), intracellular pH (pHi), and the Na(+)-H+ antiporter in cultured vascular smooth muscle cells from rat aorta. Cells were loaded with either BCECF-AM or SBFI-AM for measurement of pHi and Na+i, respectively. Ang II (10(-6) mol/L) caused a rapid rise in Na+i followed by a progressive increase that peaked at about 10 minutes (from 11 +/- 1.5 to 16 +/- 1.5 mmol/L, P < .001), whereas Ang-(1-7) (10(-6) mol/L) did not affect Na+i significantly (from 11.5 +/- 1.1 to 11.8 +/- 0.07 mmol/L). The effect of Ang II on Na+i was concentration dependent (delta Na+i, 5.1 +/- 0.9, 3.8 +/- 0.6, 1.6 +/- 0.6, and 0.14 +/- 0.18 mmol/L with decreasing concentrations of 10(-6), 10(-7), 10(-8), and 10(-9) mol/L, respectively). Ang II caused a brief acidification followed by an increase in pHi (from 7.34 +/- 0.03 to 7.43 +/- 0.03 after 10 minutes, P < .005), and Ang-(1-7) had no significant effect on pHi (from 7.23 +/- 0.03 to 7.23 +/- 0.03). To investigate whether pHi and Na+i changes induced by Ang II were due to cell Na+ entry via stimulation of the Na(+)-H+ antiporter, we pretreated cells with EIPA (25 mumol/L) or ouabain (2.0 ...Continue Reading

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Citations

Sep 2, 1999·Journal of Cardiovascular Pharmacology·P PickkersP Smits
Nov 19, 2008·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Kazuto NakamuraMasataka Sata
Jun 13, 2006·Biophysical Journal·Masood A Machingal, S V Ramanan
Sep 1, 2000·American Journal of Physiology. Renal Physiology·R ChenR V Paul
Apr 5, 2001·American Journal of Physiology. Cell Physiology·C Wu, C H Fry
Sep 1, 2000·Regulatory Peptides·R A SantosS P Andrade
May 19, 2021·Journal of Cardiovascular Pharmacology·Georgia AnastasiouEvangelos Liberopoulos

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