Angiotensin II and serotonin potentiate endothelin-1-induced vascular smooth muscle cell proliferation

Journal of Hypertension
T WatanabeC R Benedict

Abstract

Vascular smooth muscle cell (VSMC) proliferation induced by various growth factors has been implicated in a wide variety of pathological processes, including hypertension, atherosclerosis and restenosis after angioplasty. To investigate the interactions among well-known potent vasoconstrictor substances, endothelin-1 (ET-1), angiotensin II (Ang II), and serotonin (5-HT), on VSMC proliferation. Growth-arrested rabbit VSMCs were incubated with different concentrations of ET-1 in the absence or presence of Ang II, 5-HT, or both. VSMC proliferation was examined by increases in incorporation of [3H]thymidine into DNA and in cell number. ET-1, Ang II and 5-HT stimulated DNA synthesis in a dose-dependent manner. ET-1 had a maximal effect at a concentration of 0.5 micromol/l (259% of control), Ang II at 1 micromol/l (173%), and 5-HT at 50 micromol/l (205%). When added together, ET-1 (0.1 micromol/l) and Ang II (1 micromol/l) synergistically induced DNA synthesis (341%). When the vasoconstrictors were tested in combination, even non-mitogenic concentrations of ET-1 (0.01 nmol/l) potentiated 5-HT (5 micromol/l)-induced DNA synthesis (404%). Co-incubation of ET-1 (0.01 micromol/l) with Ang II (1 micromol/l) and 5-HT (5 micromol/l) synergi...Continue Reading

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Citations

Jun 14, 2005·Experimental Eye Research·Abigail GeorgeDavid V Pow
Jun 6, 2002·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Takuya WatanabeClaude R Benedict
Jun 17, 2006·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Miyuki YamamotoJun Nakura
Jun 24, 2004·Expert Opinion on Investigational Drugs·Sheila A Doggrell
Jun 15, 2004·European Journal of Pharmacology·Reinoud GosensS Adriaan Nelemans
Feb 13, 2018·Archives of Physiology and Biochemistry·Marko StojanovićMiroslav Radenković
Dec 17, 2004·American Journal of Physiology. Cell Physiology·Amy K L BanesMario B Marrero
Jan 14, 2004·Canadian Journal of Physiology and Pharmacology·Harjot K SainiNaranjan S Dhalla
Nov 8, 2013·American Journal of Physiology. Cell Physiology·Subha BhaskaranAmy Banes-Berceli
Feb 24, 2004·Cardiovascular Drug Reviews·Harjot K SainiNaranjan S Dhalla

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