PMID: 8469777Mar 19, 1993Paper

Angiotensin II binding sites on micro-organisms contaminating cell cultures

Regulatory Peptides
S WhitebreadM de Gasparo

Abstract

An angiotensin II (Ang II) binding site, distinct from AT1 and AT2, has been found in cell cultures of rat aortic smooth muscle and rat glomerular mesangium. It is characterized by a high affinity for Ang II (Kd 0.75 +/- 0.13 nM) and Ang I (Ki 0.72 +/- 0.12 nM), but a very low affinity for Ang III (Ki 31 +/- 5 microM). Ang(1-7) (Ki 1.01 +/- 0.26 nM) and Ang(1-6) (Ki 4.54 +/- 0.24 nM) are very selective for this site, with affinities more than 150- and 10,000-fold greater, respectively, than for AT1 or AT2. The selective angiotensin receptor subtype ligands losartan and L-158,809 (AT1), PD 123319 and CGP 42112A (AT2) were inactive. Binding to this site was abolished after the cells had been treated with the antibiotic mixture BM-Cyclin, suggesting that the site is located not on the cells, but on a cell culture contaminant. This has been identified as Acholeplasma laidlawii. Caution should therefore be exercised when interpreting Ang II-related data obtained from cells that have not been checked for Mollicute contamination.

References

Aug 1, 1992·Journal of Hypertension·T InagamiH Furuta
Jun 1, 1992·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·H Pfützner, P Otto
May 29, 1992·Biochemical and Biophysical Research Communications·M Q Ye, D P Healy
May 29, 1992·Biochemical and Biophysical Research Communications·H SasamuraV J Dzau
Jun 15, 1992·Biochemical and Biophysical Research Communications·S S KakarJ D Neill
Jan 1, 1990·Journal of Cardiovascular Pharmacology·M de GasparoB Kamber
Sep 30, 1991·Biochemical and Biophysical Research Communications·C BergwitzH Jüppner
Jan 15, 1992·Biochemical and Biophysical Research Communications·S Chaki, T Inagami
Dec 31, 1991·Biochemical and Biophysical Research Communications·S E WhitebreadM de Gasparo
Aug 16, 1990·Biochemical and Biophysical Research Communications·I H ChowdhuryN Yamamoto
Nov 30, 1989·Biochemical and Biophysical Research Communications·A T ChiuA L Johnson
Aug 30, 1989·Biochemical and Biophysical Research Communications·S WhitebreadM de Gasparo

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