Angiotensin II depolarizes podocytes in the intact glomerulus of the Rat

The Journal of Clinical Investigation
J GloyHermann Pavenstädt

Abstract

The aim of this study was to examine the effects of angiotensin II (Ang II) on cellular functions of rat podocytes (pod) in the intact freshly isolated glomerulus and in culture. Membrane voltage (Vm) and ion currents of pod were examined with the patch clamp technique in fast whole cell and whole cell nystatin configuration. Vm of pod was -38+/-1 mV (n = 86). Ang II led to a concentration-dependent depolarization of pod with an ED50 of 10(-8) mol/liter. In the presence of Ang II (10(-7) mol/liter, n = 20), pod depolarized by 7+/-1 mV. In an extracellular solution with a reduced Cl- concentration of 32 mmol/liter, the effect of Ang II on Vm was significantly increased to 14+/-4 mV (n = 8). The depolarization induced by Ang II was neither inhibited in an extracellular Na+-free solution nor in a solution with a reduced extracellular Ca2+ (down to 1 micromol/liter). Like Ang II, the calcium ionophore A23187 (10(-5) mol/liter, n = 9) depolarized pod by 10+/-2 mV, whereas forskolin (10(-5) mol/liter), 8-(4-chlorophenylthio)-cAMP and N2,2'-o-dibutyryl-cGMP (both 5 x 10(-4) mol/liter) did not alter Vm of pod. The angiotensin 1 receptor antagonist losartan (10(-7) mol/liter) completely inhibited the Ang II-induced (10(-7) mol/liter) de...Continue Reading

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Citations

Sep 6, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hsiang-Hao HsuHermann Pavenstädt
Jan 8, 2008·Current Hypertension Reports·Jochen Reiser, Peter Mundel
Feb 19, 2013·Molecular and Cellular Endocrinology·Ravi Nistala, Adam Whaley-Connell
Dec 4, 2003·The Journal of Laboratory and Clinical Medicine·Liming WangRobert F Spurney
Sep 15, 1998·Kidney International. Supplement·J GloyH Pavenstädt
Nov 25, 2003·Kidney International·Arrigo Schieppati, Giuseppe Remuzzi
Mar 17, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Akihiro ShimizuTaiji Matsusaka
Sep 24, 1999·Current Opinion in Nephrology and Hypertension·W Kriz, K V Lemley
May 9, 2001·Current Opinion in Nephrology and Hypertension·K EndlichR Witzgall
Nov 28, 2007·Annual Review of Pathology·Christopher KwohAndrey Shaw
Aug 8, 2008·American Journal of Physiology. Renal Physiology·Moin A SaleemPeter W Mathieson
Oct 8, 2011·Journal of the American Society of Nephrology : JASN·Anna Greka, Peter Mundel
Nov 8, 2011·Annual Review of Physiology·Anna Greka, Peter Mundel
May 6, 2015·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Janina Müller-Deile, Mario Schiffer
Mar 22, 2014·Kidney International·Daria V IlatovskayaAlexander Staruschenko
Sep 18, 1997·Kidney International·A HengerH Pavenstädt
Jan 16, 2008·Kidney International·M N Ghayur, L J Janssen
Jul 2, 2011·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Ana Diez-SampedroAlessia Fornoni
Apr 9, 2011·Biochemical and Biophysical Research Communications·Daria V IlatovskayaAlexander Staruschenko
Aug 30, 2008·The Mount Sinai Journal of Medicine, New York·Alin StirbanDiethelm Tschoepe
Sep 17, 2013·Journal of Cellular Physiology·Marc AndersonStuart E Dryer
Sep 11, 2012·Seminars in Nephrology·Dirk Oliver WennmannHermann Pavenstädt
Sep 11, 2012·Seminars in Nephrology·Anna Greka, Peter Mundel
May 9, 2012·PloS One·Sheila HarrisChryso Kanthou
Jul 1, 2004·Expert Review of Cardiovascular Therapy·Norberto PericoGiuseppe Remuzzi
Jun 19, 2018·International Journal of Molecular Sciences·Laura K SchenkKarl Kunzelmann
May 16, 2002·Microscopy Research and Technique·Hermann Pavenstädt, Martin Bek
Jan 19, 2007·Journal of the American Society of Nephrology : JASN·Noriyuki HiramatsuYoshihisa Nojima
Jan 7, 2006·Journal of the American Society of Nephrology : JASN·Michelle P WinnJohn P Middleton
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Jun 22, 2006·American Journal of Physiology. Renal Physiology·Adam T Whaley-ConnellJames R Sowers
Feb 15, 2015·The Journal of Pharmacology and Experimental Therapeutics·Ina Maria SchießlHayo Castrop
Aug 5, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura K SchenkBeate Vollenbröker
Sep 13, 2019·Journal of the American Society of Nephrology : JASN·Kazunori InoueShuta Ishibe
Jan 12, 2020·Journal of the American Society of Nephrology : JASN·Julia Binz-LotterMatthias J Hackl
Dec 31, 2002·Physiological Reviews·Hermann PavenstädtMatthias Kretzler
Jun 3, 2000·American Journal of Physiology. Renal Physiology·J GloyH Pavenstädt
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