Angiotensin II differentially regulates Morg1 expression in kidney cells

American Journal of Nephrology
Tzvetanka BondevaGunter Wolf

Abstract

The mitogen-activated protein kinase organizer 1 (Morg1) belongs to the WD-40 repeat protein family and is a scaffold molecule for the extracellular regulated kinase signaling pathway. Morg1 also binds to prolyl-hydroxylase 3 (PHD3) and regulates the hypoxia-inducible factor-1α (HIF-1α) expression via PHD3 stabilization. Morg1 has been detected in the kidney as well as in other cell tissues but its expression in renal cells has not been well investigated. It has been widely shown that angiotensin II (ANG II) mediates renal damage. We have previously shown that ANG II downregulates the expression of PHD3 in PC12 cells. The aim of this study was to analyze whether ANG II regulates Morg1 expression in mouse mesangial cells (MMC), mouse proximal tubular cells (MTC) and in differentiated podocytes. The correlation between the expression of Morg1 and PHD3 activity was also addressed. Effect of ANG II on the Morg1 mRNA expression level was assessed by real-time PCR. Morg1 and HIF-1α cellular localization was analyzed by immunohistochemistry. HIF-1α promoter activity was investigated using a reporter gene system. PHD3 hydroxylase activity test was measured with a hydroxylation-coupled decarboxylation assay. ANG II differentially regula...Continue Reading

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Citations

Jan 24, 2015·European Journal of Clinical Investigation·Ivonne Loeffler, Gunter Wolf
Jul 7, 2015·PloS One·Damien MaggioraniBénédicte Buffin-Meyer
Sep 14, 2013·Molecular Endocrinology·Tzvetanka BondevaGunter Wolf
Jan 1, 2015·American Journal of Physiology. Renal Physiology·Ivonne Loeffler, Gunter Wolf

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