Angiotensin II increases matrix metalloproteinase 2 expression in human aortic smooth muscle cells via AT1R and ERK1/2

Experimental Biology and Medicine
Chunmao WangQian Chang

Abstract

Increased levels of angiotensin II (Ang II) and activated matrix metalloproteinase 2 (MMP-2) produced by human aortic smooth muscle cells (human ASMCs) have recently been implicated in the pathogenesis of thoracic aortic aneurysm (TAA). Additionally, angiotensin II type 1 receptor (AT1R)-mediated extracellular signal-regulated kinase (ERK)1/2 activation contributes to TAA development in Marfan Syndrome. However, there is scant data regarding the relationship between Ang II and MMP-2 expression in human ASMCs. Therefore, we investigated the effect of Ang II on MMP-2 expression in human ASMCs and used Western blotting to identify the Ang II receptors and intracellular signaling pathways involved. Reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence data demonstrated that Ang II receptors were expressed on human ASMCs. Additionally, Ang II increased the expression of Ang II type 2 receptor (AT2R) but not AT1R at both the transcriptional and translational levels. Furthermore, Western blotting showed that Ang II increased MMP-2 expression in human ASMCs in a dose- and time-dependent manner. This response was completely inhibited by the AT1R inhibitor candesartan but not by the AT2R blocker PD123319. In add...Continue Reading

References

Jun 1, 1995·Journal of Vascular Surgery·G JohanssonJ Swedenborg
Mar 10, 2001·Nature·L Chang, M Karin
Jan 27, 2005·Biochemical and Biophysical Research Communications·Maren LuchtefeldBernhard Schieffer
Sep 21, 2006·The Journal of Pharmacology and Experimental Therapeutics·Márcio A F de Godoy, Satish Rattan
Aug 7, 2007·Biochemical Pharmacology·Joseph D Raffetto, Raouf A Khalil
Apr 3, 2009·Biochemical and Biophysical Research Communications·Eugenio JiménezMercedes Montiel
Apr 21, 2011·Pharmacology & Therapeutics·Els MoltzerA H Jan Danser
Jan 4, 2012·Annals of the New York Academy of Sciences·Dennis BruemmerDebra L Rateri
Aug 25, 2012·Journal of the American College of Surgeons·Abhijit GhoshGilbert R Upchurch

❮ Previous
Next ❯

Citations

Feb 2, 2016·Biochemical and Biophysical Research Communications·Yulong DingHongyan Diao
Dec 6, 2017·General Thoracic and Cardiovascular Surgery·Ayman A SaeyeldinJohn A Elefteriades
Jan 20, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Austin C BoeseMilton H Hamblin
Feb 13, 2019·Herz·N E H Dinesh, D P Reinhardt
Aug 18, 2017·Molecular Pharmacology·Maroua JalouliDarren E Richard
Mar 19, 2016·European Journal of Clinical Investigation·Menno E GroeneveldKak K Yeung
Oct 11, 2017·Cardiovascular Research·Tlili BarhoumiErnesto L Schiffrin
Jul 28, 2020·Journal of Cellular Physiology·Weiqiang WuShu Lin
Jul 6, 2019·The Journal of Thoracic and Cardiovascular Surgery·Nicole M GensickeJoseph W Turek
Apr 22, 2021·Nature Reviews. Nephrology·Justyna WozniakAndreas Ludwig
Aug 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Carolina D Avila-MesquitaChristiane Becari
Oct 27, 2021·European Journal of Clinical Investigation·Karlijn B RomboutsKak Khee Yeung

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection
protein assay
PCR
confocal microscopy

Software Mentioned

Quantity One

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aortic Aneurysm

An aortic aneurysm is the weakening and bulging of the blood vessel wall in the aorta. This causes dilatation of the aorta, which is usually asymptomatic but carries the risk of rupture and hemorrhage. Find the latest research on aortic aneurysms here.

Cardiac Aneurysm

Aneurysm refers to a bulge of the wall or lining of a vessel commonly occurring in the blood vessels at the base of the septum or within the aorta. In the heart, it usually arises from a patch of weakened tissue in a ventricular wall, which swells into a bubble filled with blood. Discover the latest research on cardiac aneurysm here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.