PMID: 9419814Jan 7, 1998Paper

Angiotensin II-induced inositol phosphate generation is mediated through tyrosine kinase pathways in cardiomyocytes

Cellular Signalling
V Goutsouliak, S W Rabkin

Abstract

The objective of this study was to determine whether the G-protein-linked angiotensin II receptor mediated inositol phosphate production involves a tyrosine phosphorylation (tyr phos) dependent pathway in the heart. Cardiomyocytes, in culture, from 7-day-old chick embryonic hearts were incubated with myo [3H] inositol for 18-24 h. Cells were incubated with LiCl to inhibit inositol 1-phosphate phosphatase and allow accumulation of inositol phosphates with angiotensin II (ang II) treatment. Inositol fractions were separated on column chromatography. Ang II produced significant (p < 0.01) increases of InsP1, InsP2, and InsP3, within 1 min of treatment of cardiomyocytes. Tyrosine kinase inhibition with genistein significantly (p < 0.05) reduced ang II induced inositol phosphate production. This did not occur with the analogue diazdien that is a very weak inhibitor of tyrosine kinase. The ability of ang II to induce tyr phos was demonstrated in whole cell lysates of cardiomyocytes immunoprecipitation with anti-P-Tyr antibodies. Genistein blunted this action of ang II. The rapid activation of a tyr phos dependent pathway by ang II was demonstrated by the similar time course of tyr phos of two different cardiac proteins, 70 and 195 kD...Continue Reading

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Citations

Feb 25, 2000·European Journal of Pharmacology·A Berts, K P Minneman
Mar 3, 2010·Progress in Biophysics and Molecular Biology·Joon-Chul KimSun-Hee Woo
Jun 27, 2006·Journal of Cellular and Molecular Medicine·Melissa R DentP S Tappia
Sep 18, 2002·Journal of Molecular and Cellular Cardiology·Natalia N PetrashevskayaArnold Schwartz
Apr 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Yakhin Shimoni, Xiu-Fang Liu
Mar 29, 2001·Physiological Reviews·G Vassort
Nov 16, 2001·American Journal of Physiology. Heart and Circulatory Physiology·C BerryD G Johns
Jan 5, 2006·Canadian Journal of Physiology and Pharmacology·Georges E HaddadSonya K Sobrian
Mar 27, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jeremy SandgrenJeffrey L Segar
Oct 16, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Georges E HaddadKrista N Blackwell

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